Rapid fabrication of a microdevice with concave microwells and its application in embryoid body formation |
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| Authors: | Xu Youchun Xie Fengbo Qiu Tian Xie Lan Xing Wanli Cheng Jing |
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| Institution: | 1Medical Systems Biology Research Center, Tsinghua University, Beijing 100084, China;2Department of Biomedical Engineering, Tsinghua University, Beijing 100084, China;3National Engineering Research Center for Beijing Biochip Technology, Beijing 102206, China;4The State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China |
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| Abstract: | Here, we report a novel method for the fabrication of polydimethylsiloxane microdevices with complicated 3-D structures, such as concave and crater shapes, using an easily machined polymethyl methacrylate mold combined with a one-step molding process. The procedure presented here enables rapid preparation of complex 3-D microstructures varying in shape and dimensions. To regulate embryoid body (EB) formation, we fabricated a microfluidic device with an array of concave microwells and found that EBs growing in microwells maintained their shape, viability, and a high degree of homogeneity. We believe that this novel method provides an alternative for rapid prototyping, especially in fabricating devices with curved 3-D microstructures. |
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