Gravity-oriented microfluidic device for uniform and massive cell spheroid formation |
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| Authors: | Lee Kangsun Kim Choong Young Yang Jae Lee Hun Ahn Byungwook Xu Linfeng Yoon Kang Ji Oh Kwang W |
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| Institution: | 1SMALL (Sensors and MicroActuators Learning Lab), Department of Electrical Engineering, The State University of New York at Buffalo, Buffalo, New York 14260, USA;2Nano-Bioresearch Center, Korea Institute of Science and Technology (KIST), 39-1 Hawolgok Dong, Songbuk Gu 136-791, Seoul, Korea;3Department of Physiology and Biophysics, The State University of New York at Buffalo, Buffalo, New York 14260, USA |
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| Abstract: | We propose a simple method for forming massive and uniform three-dimensional (3-D) cell spheroids in a multi-level structured microfluidic device by gravitational force. The concept of orienting the device vertically has allowed spheroid formation, long-term perfusion, and retrieval of the cultured spheroids by user-friendly standard pipetting. We have successfully formed, perfused, and retrieved uniform, size-controllable, well-conditioned spheroids of human embryonic kidney 293 cells (HEK 293) in the gravity-oriented microfluidic device. We expect the proposed method will be a useful tool to study in-vitro 3-D cell models for the proliferation, differentiation, and metabolism of embryoid bodies or tumours. |
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