Microcalorimetry studies on the antibacterial effect of crude monkshood polysaccharide |
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| Authors: | Gui-mei Lin Lian Liu Wei Shao |
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| Institution: | (1) School of Pharmaceutical Science, Shandong University, 44 West Culture Road, Jinan, 250012, Shandong, People’s Republic of China; |
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| Abstract: | In this paper, crude monkshood polysaccharide was isolated from Radix Aconiti Lateralis Preparata. The effects of crude monkshood
polysaccharide on Escherichia coli and Staphylococcus aureus were investigated by microcalorimetry. The power-time curves of the bacterial growth at various concentrations (c) of crude monkshood polysaccharide were plotted with a TAM air isothermal microcalorimeter at 37 °C. The growth rate constant
(μ), inhibitory ratio (I), peak-height (P
m), and peak-time (t
m) were calculated. From the data, the relationship between μ and c also was established. The growth rate constant μ decreased with the increasing concentrations of crude monkshood polysaccharide.
Moreover, P
m reduced and t
m increased with increasing concentrations. The experimental results revealed that crude monkshood polysaccharide had inhibitory
activity towards S. aureus and E. coli. Results obtained from our study strongly suggest that microcalorimetry is a fast, simple, and more sensitive technology
that can be easily performed to study the effect of drugs on bacteria. |
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| Keywords: | Crude monkshood polysaccharide Microcalorimetry Escherichia coli Staphylococcus aureus Inhibitory |
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