| 溴氰菊酯对大鼠小脑皮质组织中HO和ATPase活性的影响 |
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| 引用本文： | 王爱珠,唐敏贤,林明道,钟秀英.溴氰菊酯对大鼠小脑皮质组织中HO和ATPase活性的影响[J].宜春学院学报,2012,34(8):102-104. |
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| 作者姓名： | 王爱珠 唐敏贤 林明道 钟秀英 |
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| 作者单位： | 广州市荔湾区茶沼街社区卫生服务中心,广州,510375 |
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| 摘 要： | 目的:研究溴氰菊酯(DM)对SD大鼠小脑皮质组织中血红素加氧酶(HO)的活性和ATPase活性的影响。方法:(1)成年雄性SD大鼠共40只,其中20只用于血红素加氧酶(HO)的活性的测定,另20只用于ATPase活力测定,均随机分为对照组和DM染毒实验组,实验组中大鼠72小时内给溴氰菊酯(1/20LD50)三次,对照组为相同剂量的玉米油,分别检测小脑皮质组织HO活性和ATPase活性。(2)成年雄性SD大鼠30只,同样方法取出小脑皮质,制成切片,随机分为对照组(相同剂量的玉米油)和5个不同浓度的DM染毒试验组(2×10-7mmol/L、2×10-6mmol/L、2×10-5mmol/L、2×10-4mmol/L、2×10-3mmol/L),测定ATPase活性。结果:整体染毒小脑皮层组织HO活性为对照组的240.39%(p﹤0.05),Na+,K+-ATPase活性为对照组的131.47%(p﹤0.05),Ca2+-ATPase活性增加没有统计学意义,Mg2+-ATPase有显著的抑制作用(P<0.05),为对照组的79.91%。体外试验发现只有达到10-3mmol/L数量级的DM对温育小脑皮质切片组织中的Mg2+-ATPase活性才有显著的抑制作用(P<0.05);且无论何种浓度均未发现上述体内染毒时对Na+,K+-ATPase活性的增加作用。结论:DM可增加大鼠小脑皮质的HO活性,体内体外染毒DM对三种ATPase活性的影响并不一致。
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| 关 键 词： | 溴氰菊酯 血红素加氧酶 ATPase 活性 |
The Influence of the DM on HO and ATPase Activity in Rat Cerebellar Cortex |
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| WANG Ai-zhu,TANG Ming-xian,LIN Ming-dao,ZHONG Xiu-ying.The Influence of the DM on HO and ATPase Activity in Rat Cerebellar Cortex[J].Journal of Yichun University,2012,34(8):102-104. |
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| Authors: | WANG Ai-zhu TANG Ming-xian LIN Ming-dao ZHONG Xiu-ying |
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| Institution: | (Chajiao Street Community Health Service Center,Guangzhou 510375,China) |
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| Abstract: | Objective:To study the effects of deltamethrin(DM) on heme oxygenase(HO) activity and ATPase activity in SD rat’s cerebellar cortex tissue.Methods:(1) There were 40adult male SD rats,of which 20 was only used for heme oxygenase(HO) activity determination,and the other 20 were used only for ATPase activity determination.They were randomly divided into control group and DM experimental group.The rats in experimental group were exposed to deltamethrin(1/20 LD50 three times within 72 hours,while control group were given corn oil with the same dose.They both were used to detect HO activity and ATPase activity in the cerebellar cortex tissue.(2) Cerebellar cortexes were removed from 30 adult male SD rats in the same way and were cut into slices.They were randomly divided into control group(corn oil with the same dose) and 5 experimental groups with different concentrations,which were exposed to DM(2×10-7mmol/L,2×10-6mmol/L,2×10-5mmol/L,2×10-4mmol/L,2×10-3mmol/L).ATPase activity was determined.Results:The HO activity in cerebellar cortical tissue after overall exposure was 240.39% in comparison with the control group(P<0.05).Na+,the activity of K+-ATPase was 131.47% in comparison with the control group(P<0.05).Increased Ca2+-ATPase activity was not statistically significant.Mg2+-ATPase was significantly inhibited(P<0.05),as compared to a control group of 79.91%.In vitro tests have found only when DM reached 10-3mmol/L orders of magnitude can it inhibit Mg2+-ATPase in cerebellar cortex slices significantly(P<0.05);and whatever the concentration was,increased role in Na+ and K+-ATPase activity was not found in the in vivo exposure.Conclusion:DM can increase the HO activity in rat cerebellar cortex.Effects of DM exposed in vitro and in vivo on three kinds of ATPase activity are not consistent. |
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| Keywords: | Deltamethrin Heme Oxygenase Activity ATPase |
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