| 强脉冲电场致细胞膜电穿孔的实验研究 |
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| 作者姓名： | 肖华娟 严萍 牟群 |
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| 作者单位： | 1. 中国科学院电工研究所 北京 100080;
2. 中国科学院研究生院 北京 100049 |
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| 基金项目： | 国家自然科学基金项目 (50447007)资助 |
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| 摘 要： | 采用肝癌细胞HePG2作为靶细胞,罗丹明偶联葡聚糖为荧光探针,荧光分光光度计作为检测设备,试验研究细胞膜电穿孔与电脉冲参数之间的关系.试验中发现随着电场强度或脉冲宽度的增加,样品荧光强度增加,细胞膜穿孔率相应增强;同时发现细胞膜电穿孔效应具有累积效应
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| 关 键 词： | 细胞膜电穿孔 强脉冲电场 生物效应 |
| 收稿时间： | 2004-03-18 |
| 修稿时间： | 2004-09-15 |
The Experimental Study of Electroporation of Cell Membrane Induced by Pulsed Electric Field of High Intensity |
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| Authors: | XIAO Hua-Juan YAN Ping MU Qun |
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| Institution: | 1. Institute of Electrical Engineering, Chinese Academy of Sciences, Beijing 100080, China;
2. Graduate School of the Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | In this paper, cell membrane electroporation induced by pulsed electric field is studied by using liver cancer cells as target cells, Rhodamine Bisothiocyanate-Dextran as fluorescent probes and fluorescence spectrophotometer as measure equipment. The experiments have shown that when the pulse intensity or pulse width increases, the fluorescent intensity will increase accordingly, so will the rate of electroporation of cell membranes. Also we find that electroporation of cell membrane presents memory effects. |
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| Keywords: | electroporation of cell membrane high intense pulsed electric field biological effect |
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