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荷叶离褶伞摇瓶发酵条件研究
引用本文:席亚丽,王治江,魏生龙,张汉燚.荷叶离褶伞摇瓶发酵条件研究[J].河西学院学报,2011,27(2):68-74,57.
作者姓名:席亚丽  王治江  魏生龙  张汉燚
作者单位:河西学院食用菌研究所,甘肃,张掖,734000
基金项目:科技部农业科技成果转化基金
摘    要:采用摇瓶发酵法对荷叶离褶伞(Lyophyllum decastes)发酵条件进行研究.结果表明:荷叶离褶伞菌丝体生长的最适碳源为玉米面;最适氮源为麸皮;最适碳氮比为16:1;最佳培养基配方为玉米面200g/L,蔗糖15g/L,麸皮35g/L,酵母膏1.5 g/L;最适发酵条件:pH值7.0,温度26℃,接种量10%,转速160r/min;稳定生长期为接菌后6-8d.

关 键 词:荷叶离褶伞  摇瓶发酵  发酵条件

Study on Fermentation Conditions of Lyophyllum Decastes in Shake Flask
Xi Ya-li,Wang Zhi-jiang,Wei Sheng-long,Zhang Han-yi.Study on Fermentation Conditions of Lyophyllum Decastes in Shake Flask[J].Journal of Hexi University,2011,27(2):68-74,57.
Authors:Xi Ya-li  Wang Zhi-jiang  Wei Sheng-long  Zhang Han-yi
Institution:yi(Mushroom Research Institute,Hexi University,Zhangye 734000,China)
Abstract:In this study,fermentation conditions of Lyophyllum decastes in a shake flask are explored.The optimum carbon source is corn flour,the optimum nitrogen source is wheat bran,the most suitable ratio of carbon to nitrogen is 16:1;the best formula is as follow:corn flour 200g/L,sucrose 15g/L,wheat bran 35g/L,and yeast extract 1.5g/L.The optimum conditions include pH 7.0,temperature 26℃;inoculation quantity 10%,shaking speed 160r/min,incubation time 6-8 days.
Keywords:Lyophyllum decaste  Shake flask  Fermentation conditions
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