A hydrodynamic focusing microchannel based on micro-weir shear lift force |
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| Authors: | Ruey-Jen Yang Hui-Hsiung Hou Yao-Nan Wang Che-Hsin Lin Lung-Ming Fu |
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| Institution: | 1.Department of Engineering Science, National Cheng Kung University, Tainan 70101, Taiwan;2.Department of Vehicle Engineering, National Pingtung University of Science and Technology, Pingtung 912, Taiwan;3.Department of Mechanical and Electro-Mechanical Engineering, National Sun Yat-sen University, Kaohsiung 804, Taiwan;4.Department of Materials Engineering, National Pingtung University of Science and Technology, Pingtung 912, Taiwan |
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| Abstract: | A novel microflow cytometer is proposed in which the particles are focused in the horizontal and vertical directions by means of the Saffman shear lift force generated within a micro-weir microchannel. The proposed device is fabricated on stress-relieved glass substrates and is characterized both numerically and experimentally using fluorescent particles with diameters of 5 μm and 10 μm, respectively. The numerical results show that the micro-weir structures confine the particle stream to the center of the microchannel without the need for a shear flow. Moreover, the experimental results show that the particles emerging from the micro-weir microchannel pass through the detection region in a one-by-one fashion. The focusing effect of the micro-weir microchannel is quantified by computing the normalized variance of the optical detection signal intensity. It is shown that the focusing performance of the micro-weir structure is equal to 99.76% and 99.57% for the 5-μm and 10-μm beads, respectively. Overall, the results presented in this study confirm that the proposed microcytometer enables the reliable sorting and counting of particles with different diameters. |
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