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粉花月见草中总黄酮含量的分析测定
引用本文:杨再波,张炜.粉花月见草中总黄酮含量的分析测定[J].黔南民族师范学院学报,2011(6):1-3.
作者姓名:杨再波  张炜
作者单位:贵州省黔南民族师范学院化学与化工系;
基金项目:贵州省教育厅2010年度自然科学研究基金资助项目(黔教科2010094); 贵州省黔南民族师范学院2009年度重点资助科研项目(QNSY0915)
摘    要:目的:测定粉花月见草中总黄酮的含量。方法:以无水乙醇作为回流提取溶剂,在375nm处用紫外可见分光光度法测定粉花月见草中总黄酮的含量。结果:测得粉花月见草中总黄酮的含量为1.4435mg/g,精密度RSD=0.14%(n=5),回收率为90.87%~98.98%。结论:该方法操作简便、准确、可靠,可用于植物中总黄酮的含量测定。

关 键 词:粉花月见草  山奈酚  紫外分光光度法  含量测定

On the Determination of the content of Total Flavonoids in Oenothera Rosea
YANG Zaibo,ZHANG Wei.On the Determination of the content of Total Flavonoids in Oenothera Rosea[J].Journal of Qiannan Normal College of Nationalities,2011(6):1-3.
Authors:YANG Zaibo  ZHANG Wei
Institution:YANG Zaibo,ZHANG Wei(Dept.,of Chemistry and Chemical Engineering,Qiannan Normal University for Nationalities,Duyun 558000,Chian)
Abstract:Objective: To determine the content of total flavonoids in Oenothera rosea.Methods: Ethanol is applied as the extracting solvent,at 375nm with UV-visible spectrophotometry to determine the content of total flavonoids in Oenothera rosea.Results: The content of total flavonoids 1.4435mg / g,the precision RSD = 0.14%(n = 5),the rate of recovery 90.87%~98.89%.Conclusion: The method is simple,accurate,reliable,and can be applied to determine the content of total flavonoids in plants.
Keywords:oenothera rosea  kaempferol  UV-visible spectrophotometry  the determination of content  
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