| 血管内皮生长因子逆转录病毒表达载体的构建 |
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| 引用本文： | 王进,师杨.血管内皮生长因子逆转录病毒表达载体的构建[J].周口师范学院学报,2012,29(2):79-80,139. |
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| 作者姓名： | 王进 师杨 |
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| 作者单位： | 周口师范学院生命科学系,河南周口,466001 |
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| 摘 要： | 将质粒pcDNA3．1-VEGF双酶切后亚克隆至逆转录病毒载体pLXSN，并用酶切及测序等手段进行鉴定．而后利用脂质体转染包装细胞pA317，并检测病毒滴度．结果表明：成功构建了VEGF基因的重组逆转录病毒表达载体．
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| 关 键 词： | VEGF 逆转录病毒载体 病毒滴度 |
Construction of retroviral vector containing VEGF gene |
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| WANG Jin,SHI Yang.Construction of retroviral vector containing VEGF gene[J].Journal of Zhoukou Normal University,2012,29(2):79-80,139. |
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| Authors: | WANG Jin SHI Yang |
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| Institution: | (Department of Life Science,Zhoukou Normal University,Zhoukou 466001,China) |
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| Abstract: | According to compatible restriction sites,we digest the plasmid pcDNA3.1-VEGF and pLXSN and connect them together,then perform enzyme cutting and sequence analysis to identify the connection.Lipofectamine was used to mediate recombinant plasmid pLXSN-VEGF transfection of pA317 packaging cells,collect virus-containing medium from packaging cells,infect NIH3T3 cells to determine viral titer.The results indicate that retroviral vector pLXSN-VEGF was successfully established. |
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| Keywords: | VEGF retroviral vector viral titer |
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