| 酶辅助法提取大青叶中总黄酮及抗氧化性研究 |
| |
| 引用本文： | 崔旭,李会端.酶辅助法提取大青叶中总黄酮及抗氧化性研究[J].楚雄师范学院学报,2014,29(6):46-52. |
| |
| 作者姓名： | 崔旭 李会端 |
| |
| 作者单位： | 楚雄师范学院化学与生命科学学院,云南楚雄,675000 |
| |
| 基金项目： | 云南省应用基础研究项目，楚雄师范学院科学研究基金项目 |
| |
| 摘 要： | 本论文采用酶辅助法提取大青叶中的总黄酮,先用纤维素酶酶解,再用乙醇回流提取大青叶中总黄酮。分别固定乙醇提取液的浓度为70%,料液比为1∶20g∕mL,回流时间为2h。初步探究了纤维素酶浓度、酶解pH、酶解温度、酶解时间四个单因素对大青叶中黄酮提取率的影响。设计正交实验确定酶辅助法提取大青叶中总黄酮的较佳条件为:纤维素酶浓度为4U/mL、酶解pH=4.5、酶解温度为40℃、酶解时间1.5h,总黄酮提取率高达2.85%。另外还研究了大青叶总黄酮提取液对羟自由基的清除活性,与BHT的抗氧化剂活性作对照实验,结果表明大青叶中的总黄酮对羟自由基有较强的清除活性。
|
| 关 键 词： | 酶辅助法 大青叶 总黄酮 提取率 清除活性 羟自由基 |
Enzyme-assisted Extraction of Total Flavonoids from Folium Isatidis and Study on Antioxidant Effects |
| |
| CUI Xu,LI Huiduan.Enzyme-assisted Extraction of Total Flavonoids from Folium Isatidis and Study on Antioxidant Effects[J].journal of chuxiong normal university,2014,29(6):46-52. |
| |
| Authors: | CUI Xu LI Huiduan |
| |
| Institution: | CUI Xu ＆ LI Huiduan (School of Chemistry and Life Science, Chuxiong Normal University, Chuxiong, 675000, Yunnan Province) |
| |
| Abstract: | Enzyme - assisted extraction of total flavonoids from folium Isatidis was studied in this pa-per. Frist enzymatic hydrolysis by cellulase, and then reflux by ethanol to extract total flavonoids. Ethanolconcentration of 70% , solid -liquid ratio of I ：20g/mL and reflux time of 2h were fixed. The preferred ex-traction conditions were determined by single factor experiment and orthogonal experiment. The experimentreveals that ： cellulase concentration of 4U/mL, enzymatic hydrolysis pH of 4.5, enzymatic hydrolysis tem-perature of 40℃, and enzymatic hydrolysis time of 1.5 h. The extraction ratio of total flavonoids reached upto 2.85 % by using spectrophotometry. Antioxidant effects of flavonoids and BHT were measured to removehydroxyl radicals by comparative experiments. Antioxidant experiments showed that flavonoids from foliumIsatidis showed a strong scavenging effect on hydroxyl radicals. |
| |
| Keywords: | enzyme - assisted method folium Isatidis total flavonoids extraction ratio scavengingeffect hydroxyl radicals |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
