| 固相微萃取-液相色谱联用在生物分析中的新策略 |
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| 引用本文： | 冯蕾,孟庆青,杨海莎,朱云婷,陈晓静.固相微萃取-液相色谱联用在生物分析中的新策略[J].实验技术与管理,2011(11). |
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| 作者姓名： | 冯蕾 孟庆青 杨海莎 朱云婷 陈晓静 |
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| 作者单位： | 上海交通大学分析测试中心;上海交通大学医学院;上海交通大学药学院; |
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| 基金项目： | 上海市科委技术标准专项08DZ0504700 |
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| 摘 要： | 集萃取、浓缩、解吸、进样于一体的固相微萃取(solid-phase microextraction,SPME)样品预处理技术可与高效液相色谱(HPLC)联用,非常适合复杂生物样本中的痕量非挥发性目标化合物的定性与定量分析,但存在自动化程度不高、涂层种类少、涂层稳定性差等问题。为此,整理并总结了近年改进策略的研究进展,介绍了不同的SPME萃取形式与HPLC的联用接口设计、新型涂层的制作方法和研究思路,指出了SPME-HPLC未来的发展方向是全自动化、快速、高通量、高灵敏度。
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| 关 键 词： | 固相微萃取 高效液相色谱 生物分析 接口 |
New strategies of solid-phase microextraction-high performance liquid chromatography in field of bioanalysis |
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| Feng Lei,Meng Qingqing ,Yang Haisha ,Zhu Yunting ,Chen Xiaojing.New strategies of solid-phase microextraction-high performance liquid chromatography in field of bioanalysis[J].Experimental Technology and Management,2011(11). |
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| Authors: | Feng Lei Meng Qingqing Yang Haisha Zhu Yunting Chen Xiaojing |
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| Institution: | Feng Lei1,Meng Qingqing 2,Yang Haisha 3,Zhu Yunting 3,Chen Xiaojing 3 (1.Instrumental Analysis Center,Shanghai Jiaotong University,Shanghai 200240,China,2.School of Medicine,3.School of Pharmacy,China) |
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| Abstract: | Hyphenation of solid-phase microextraction(SPME),which integrates sample extraction,concentration,desorption and injection in one step,with HPLC is widely applied to analyze non-volatile substance residues quantitatively and qualitatively.However semi-automation,lack of candidate SPME coating,instability of the coating restricted the progress and application of SPME-HPLC.Corresponding strategies and recent progress are stated in detail,including different SPME-HPLC interfaces,novel coating methods and promi... |
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| Keywords: | solid-phase microextraction(SPME) high performance liquid chromatography(HPLC) bioanalysis interface |
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