| 谷精草总黄酮纤维素酶提取工艺的研究 |
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| 引用本文： | 薛天乐,孙鹏,丁锐,杜小满.谷精草总黄酮纤维素酶提取工艺的研究[J].安徽科技学院学报,2017,31(1). |
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| 作者姓名： | 薛天乐 孙鹏 丁锐 杜小满 |
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| 作者单位： | 亳州职业技术学院 药学院,安徽 亳州,236800;亳州职业技术学院 药学院,安徽 亳州,236800;亳州职业技术学院 药学院,安徽 亳州,236800;亳州职业技术学院 药学院,安徽 亳州,236800 |
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| 基金项目： | 安徽省教育厅自然科学重点课题，亳州市中药科技创新团队 |
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| 摘 要： | 目的:研究纤维素酶法提取谷精草中总黄酮的最佳工艺。方法:采用紫外分光光度法分别考察酶用量、提取时间、料液比、提取温度对谷精草总黄酮提取率的影响,并在此基础上设计L9(34)正交试验,优选最佳提取工艺。结果:最佳条件是酶用量0.03 g,提取时间2.5 h,料液比1∶40,提取温度55℃,采用最佳工艺,总黄酮提取率可达0.879%。结论:该方法可以用于谷精草总黄酮的提取。
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| 关 键 词： | 谷精草 纤维素酶 总黄酮 提取工艺 |
Study on Extraction Technology of Total Flavones UsingCellulase from Eriocaulon Buergerianum Koern |
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| XUE Tian-le,SUN Peng,DING Rui,DU Xiao-man.Study on Extraction Technology of Total Flavones UsingCellulase from Eriocaulon Buergerianum Koern[J].Journal of Anhui Science and Technology University,2017,31(1). |
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| Authors: | XUE Tian-le SUN Peng DING Rui DU Xiao-man |
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| Abstract: | Objective:To study the optimum extraction technology of total flavonoids from Eriocaulon buergerianum Koern by cellulase method.Methods: UV spectrophotometric method was used to observe the influence of enzyme dosage, extraction time,extraction temperature and solid-liquid ratio on the extraction rate of total flavonoids from Eriocaulon buergerianum Koern.And on this basis, the L9(34) orthogonal test had been used to select the best extraction.Results: The optimum condition was enzyme dosage 0.03 g, extraction time 2.5 h, material liquid ratio 1∶40, extraction temperature 55℃, and the extraction rate of flavonoids was 0.879% by using the optimum condition.Conclusions: The method can be used for the extraction of total flavonoids from Eriocaulon buergerianum Koern. |
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| Keywords: | Eriocaulon buergerianum Koern Cellulase Total flavonoids Extraction technology |
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