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| 斑点免疫酶渗滤法检测分泌物中淋病奈瑟氏菌抗原 | |
| 引用本文： | 张勤,张新萍,徐冉行,单小云,胡野.斑点免疫酶渗滤法检测分泌物中淋病奈瑟氏菌抗原[J].金华职业技术学院学报,2001,1(2):48-49. |
| 作者姓名： | 张勤 张新萍 徐冉行 单小云 胡野 |
| 作者单位： | 1. 杭州第六人民医院,检验师,310000,杭州市 2. 杭州第六人民医院,主管检验师 3. 金华中心医院,主管检验师,321000,金华市 4. 金华职业技术学院医学院,副教授,321000,金华市 |
| 摘 要： | 目的：建立分泌物中淋病奈瑟氏菌抗原快速斑点免疫酶渗滤法(DIEFA)。方法：应用DIEFA检测分泌物中淋病奈瑟氏菌抗原，并与常规淋病奈瑟氏菌培养及间接免疫荧光染色法进行比较研究。结果：DIEFA检测分泌物中淋病奈瑟氏菌抗原敏感性高于常规培养法及间接免疫荧光染色法。整个检测过程在3h内完成。斑点色泽鲜艳，结果易于判断。结论：DIEFA敏感性高、特异性强、且简便、快速，具有实际应用价值。 |
| 关 键 词： | 斑点免疫酶渗滤法 检测 分泌物 细菌培养 淋病奈瑟氏菌抗原 |
| 修稿时间： | 2001年3月27日 |
Application of a dot-immunoenzyme filtration assay for the detection of neisseria gonorrhoeae antigen in secretion |
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| Zhang Qin,Zhang Xinping,Xu Ranxing.Application of a dot-immunoenzyme filtration assay for the detection of neisseria gonorrhoeae antigen in secretion[J].Journal of Jinhua College of Profession and Technology,2001,1(2):48-49. | |
| Authors: | Zhang Qin Zhang Xinping Xu Ranxing |
| Institution: | Zhang Qin Zhang Xinping Xu Ranxing(Hangzhou 6th people's hospital)Hu Ye(Jinhua College of rofession and Technology)Shan Xiaoyun(Jinhua central Hospital) |
| Abstract: | A dot-immunoenzyme filtration assay (DIEFA) was developed for the detection of neisseria gonorrhoeae antigen in secretion, and compared with the indirect immunofluorescence stain tech-nique (FA) the routine culture of the neisseria gonorrhoeae. This method was simple, rapid and the whole manipulation only needed 3 hours. The color of the positive dots were so bright that the results could be easily determined. Comparison this method with the conventional culture technique and FA, its sensitivity was shown to be highest than that of other methods. Its extension for practical use is valuble. |
| Keywords: | dot-immunoenzyme filtration assay (DIEFA)neisseria gonorrhoeae antigen the rou-tine culture of the neisseria gonorrhoeae Indirect immunofluorescence stain technique (IIFA) |
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