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几种泥炭藓属植物苹果酸脱氢酶同工酶研究
引用本文:张春,张以忠.几种泥炭藓属植物苹果酸脱氢酶同工酶研究[J].毕节学院学报,2012,30(8):87-90.
作者姓名:张春  张以忠
作者单位:毕节学院地理与生命科学学院,贵州毕节,551700
摘    要:采用聚丙烯酰胺凝胶电泳对泥炭藓属植物5个种10个居群的苹果酸脱氢酶同工酶进行分析,结果表明:苹果酸脱氢酶同工酶带共6条,MDH-5和MDH-6在5个种中均可见,其酶带明显,染色较深,说明酶活性较强,可以判断MDH-5和MDH-6为共有特征带。泥炭藓有酶带2条,卵叶泥炭藓有酶带5条,加萨泥炭藓有酶带6条,多纹泥炭藓有酶带4条,尖叶泥炭藓有酶带4条,通过SPSS系统聚类表明:泥炭藓属5泥炭藓植物种间存在遗传差异。

关 键 词:泥炭藓  苹果酸脱氢酶同工酶  酶带

Study on Malate Dehydrogenase lsozyme of Several Sphagnum
ZHANG Chun,ZHANG Yi-Zhong.Study on Malate Dehydrogenase lsozyme of Several Sphagnum[J].Journal of Bijie University,2012,30(8):87-90.
Authors:ZHANG Chun  ZHANG Yi-Zhong
Institution:1(School of Geography and Life Science,Bijie University,Bijie,Guizhou551700,China)
Abstract:The malate dehydrogenase isozymes of ten populations belonging to five species of Sphagnum were studied by means of polyacrylamide gel eletrophoresis(PAGE).The results showed that there were six different hands of malate dehydrogenase isozyme.Among them,MDH-5 and MDH-6 were common and characteristic bands.Sphagnum palustre L.had two bands,Sphagnum ovatum Hamp.had five bands,Sphagnum khasiaum Mitt.had six bands,Sphagnum multifibrosum Li at Zang.and Sphagnum nemoreum Scop.had four bands.Spss systems clustering showed that there were genetic differences among five different species of Sphagnum.
Keywords:Sphagnum  Dehydrogenase Isozymes  Enzyme Bands
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