| 甜樱桃砧木‘Colt’小茎尖离体再生体系的建立 |
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| 引用本文： | 侯义龙,李俊芳,张立娟,于亚军.甜樱桃砧木‘Colt’小茎尖离体再生体系的建立[J].大连大学学报,2007,28(3):53-56. |
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| 作者姓名： | 侯义龙 李俊芳 张立娟 于亚军 |
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| 作者单位： | 大连大学生命科学工作室大连大学生物工程学院 辽宁大连116622(侯义龙，张立娟，于亚军)，内蒙古农业大学 内蒙古呼和浩特010019(李俊芳) |
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| 基金项目： | 辽宁省教育厅高校科研项目（2004D202）,辽宁省科技项目（20021052）和大连大学科技发展基金项目 |
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| 摘 要： | 目的]建立甜樱桃砧木Colt的离体培养体系.方法]采取小茎尖离体培养的方法.结果]在茎尖分化培养中,合适的培养基为1/2MS+1.0 mg/L BA+0.1 mg/L IBA.在增殖培养中,经过筛选认为,最佳培养基为1/2MS+1.0 mg/L BA+0.2 mg/L IBA+0.5 mg/L GA3,增殖系数高,植株生长势旺,增殖阶段最适的pH值为5.2～6.0.最适的蔗糖浓度是2%～3%.生根培养阶段,培养基1/2MS+0.5 mg/L IBA上试管苗的生根效果最好,生根率高.结论]对茎尖培养各个阶段的多种影响因素组合进行了研究,初步建立了一套较为完整的甜樱桃砧木Colt的小茎尖培养体系.
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| 关 键 词： | 甜樱桃砧木 Colt 小茎尖离体培养 |
| 文章编号： | 1008-2395（2007）03-0053-04 |
| 修稿时间： | 2006-10-202007-04-05 |
Establishment of the Minishoot-Tip Culture System in Vitro in Sweet Cherry Rootstock Colt' |
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| HOU Yi-long, LI Jun-fang, ZHANG Li-juan, YU Ya-jun.Establishment of the Minishoot-Tip Culture System in Vitro in Sweet Cherry Rootstock Colt''[J].Journal of Dalian University,2007,28(3):53-56. |
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| Authors: | HOU Yi-long LI Jun-fang ZHANG Li-juan YU Ya-jun |
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| Abstract: | Objective]To establish the technique systems of minishoot-tip culture in Colt.Methods]The regeneration culture of minishoot-tip was used.Results]Factors influencing propagation were optimized and discussed,A relatively optimal culture system for Colt shoot-tips is established.The optimized medium for shoot-tips initial culture was 1/2MS+1.0mg/L BA+0.1 mg/L IBA.In subculture multiplification,the optimized medium was 1/2MS+1.0 mg/L BA+0.2 mg/L IBA+0.5 mg/L GA3 in which the propagation ratio is high and the vigor of trees is strong.The suitable value of pH is 5.2～6.0,Suitable density of sugar is 2%～3%.The optimized medium of rooting was 1/2MS+0.5 mg/L IBA in which the ratio of rooting was high and roots grew quickly and strongly.Conclusion]Based on the study of many factors,the systems of minishoot-tip culture in Colt was established. |
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| Keywords: | sweet cherry rootstock Colt minishoot-tip culture in vitro |
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