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玉米种子成熟过程中细胞膜脂过氧化及体内抗氧化酶活性变化的研究
引用本文:张艳红,文才艺.玉米种子成熟过程中细胞膜脂过氧化及体内抗氧化酶活性变化的研究[J].襄樊学院学报,2000,21(5):43-46.
作者姓名:张艳红  文才艺
作者单位:[1]华东理工大学生物工程学院,上海200237 [2]襄樊学院化学化工系,湖北襄樊441053
摘    要:研究了沈试-29玉米种子在成熟过程中四种酶活性的变化及膜脂过氧化产物丙二醛(MDA)含量的变化。结果表明:超氧化物歧化酶(SOD),过氧化物酶(POD)和过氧化氢酶(CAT)活性的变化一致即由乳熟期到蜡熟期活性下降,完熟期和枯熟期SOD、POD和CAT的活性又开始上升,而抗坏血酸氧化酶(AAO)的活性的变化则与此相反;MDA含量在蜡熟期上升,在完熟期和枯熟期下降。由此可见,种子的成熟过程中发生了氧化和过氧化作用,并存在活性氧的伤害。

关 键 词:玉米  超氧化物歧化酶  过氧化物酶  过氧化氢酶  抗坏血酸氧化酶  丙二醛  种子成熟  膜脂过氧化  酶活性

On Cell Membrane Lipid Peroxidation and Changes of Oxide Resistance Enzymes Activities during the Ripeness of Maize Seeds
ZHANG Yan-hong,WEN Cai-yi.On Cell Membrane Lipid Peroxidation and Changes of Oxide Resistance Enzymes Activities during the Ripeness of Maize Seeds[J].Journal of Xiangfan University,2000,21(5):43-46.
Authors:ZHANG Yan-hong  WEN Cai-yi
Abstract:Studies have been done on the changes of four kinds of enzymes activities and malondialdehyde (MDA) content during the ripening stage of maize seeds. The results indicated that the changes of activity of superoxide dismutase (SOD), peroxidase(POD) and catalase(CAT) were consistent with each other: the activity of all three kinds of enzymes decreased from milky ripeness stage to waxy ripeness stage, but increased during complete ripeness stage and withered ripeness stage, the change of the MDA content and ascorbic acid oxidase(AAO) activity were just contrary to that of SOD, POD and CAT activities. The analysis showed that the ripeness of maize seeds was associated with the toxicity of active oxygen, it has occurred oxidation and peroxidation.
Keywords:Maize ripe seeds  Superoxide dismutase(SOD): Peroxidase (POD)  Catalase(CAT): Ascorbic acid oxidase (AAO): Malondialdehyde (MDA)
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