Enantioselective assay of S(+)- and R(-)-propafenone in human urine by using RP-HPLC with pre-column chiral derivatization |
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| Authors: | Wu Yong-jiang Ma Ming-ming Zeng Su |
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| Institution: | (1) College of Pharmaceutical Sciences, Zhejiang University, 310031 Hangzhou, China |
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| Abstract: | The enantioselective assay for S(+)-and R(-)-propafenone (PPF) in human urine that developed in this work involves extraction
of propafenone from human urine and using S(+)-propafenone as internal standard, chiral derivatization with 2,3,4,6-tetra-O-β-D-glucopranosyl isothiocyanate, and quantitation by an RP-HPLC system with UV detection (γ-220 nm). A baseline separation
of propafenone enantiomers was achieved on a 5-μm reverse phase ODS column, with a mixture of methanol:water:glacial acetic
acid (25∶12∶0.02, v/v) as mobile phase. There was good linear relationship from 24.9 ng/ml to 1875.0 ng/ml for both of enantiomers.
The regression equations of the standard curves based on CS-PPF (or CR-PPF) versus ratio of AS-PPF/As (or AR-PPF/As) werey=0.0032x−0.081, (r=0.999) for S-PPF andy=0.0033x+0.0039, (r=0.998) for R-PPF, respectively. The method’s limit of detection was 12.5 ng/ml for both enantiomers, and the method’s limit
of quantitation was 28.2±0.52 ng/ml for S-PPF, 30.4±0.53 ng/ml for R-PPF (RSD<8%,n=5). The analytical method yielded average recovery of 98.9% and 100.4% for S-PPF and R-PPF, respectively. The relative standard
derivation was no more than 6.11% and 6.22% for S-PPF and R-PPF, respectively. The method enabled study of metabolism of S(+)-and
R(-)-propafenone in human urine. The results from 7 volunteers administered 150 mg racemic propafenone indicated that propafenone
enantiomers undergo stereoselective metabolism and that in the human body, S(+)-propafenone is metabolized more extensively
than R(-)-propafenone.
Project supported by the National Natural Science Foundation of China (No. 30225047) and by SRF for ROCS, SEM and Zhejiang
Provincial Natural Science Fundation (No. RC97016), China |
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| Keywords: | Enantioselective assay Propafenone Human urine Chiral derivatization High-performance liquid chromatography |
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