| 心脏分子标志物NT-proBNP在运动预适应保护效应评价中的应用 |
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| 引用本文： | 马治云,潘珊珊,申毓军,郝喆,葛珺,王凯.心脏分子标志物NT-proBNP在运动预适应保护效应评价中的应用[J].体育科学,2011,31(12):55-61. |
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| 作者姓名： | 马治云 潘珊珊 申毓军 郝喆 葛珺 王凯 |
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| 作者单位： | 上海体育学院运动科学学院,上海,200438 |
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| 基金项目： | 国家自然科学基金资助项目，上海市科学技术委员会资助项目 |
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| 摘 要： | 目的:以运动预适应(EP)诱导心肌保护效应为研究基础,以心脏分子标志物NT-proBNP为研究核心,联合应用血清NT-proBNP与血清cTnI和心肌HBFP染色,综合评价EP对力竭运动心肌的保护效应;方法:SD大鼠100只,随机分为对照组(C组)、力竭组(EE组)、运动预适应组(EP组)和运动预适应+力竭组(EP+EE组)。采用一次间歇性大强度跑台运动建立EP模型,力竭跑台运动建立急性心肌损伤模型。用ELISA法检测血清NT-proBNP浓度,CLIA法检测血清cTnI浓度,HBFP染色法观察心肌缺血缺氧状况;结果:与C组比较,EE组血清NT-proBNP浓度显著升高(P<0.05),EP组变化不明显(P>0.05);与EP组比较,EP+EE组血清NT-proBNP浓度变化不明显(P>0.05);与EE组比较,EP+EE组血清NT-proBNP浓度显著下降(P<0.05)。各组大鼠血清NT-proBNP浓度与血清cTnI浓度、心肌HBFP染色结果的变化趋势一致;结论:EP是一种非损伤性预适应方式,能明显减轻力竭运动引起的急性心肌损伤和促进心脏功能恢复。血清NT-proBNP与血清cTnI和心肌HBFP染色联合应用具有互补性,有助于综合评价EP对力竭运动心肌的保护效应。
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| 关 键 词： | 氨基末端-脑钠肽前体 运动预适应 心肌保护效应 力竭运动 鼠 动物实验 |
Application of Cardiac Molecular Marker NT-proBNP in the Assessment of Cardioprotective Effect of Exercise Preconditioning |
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| MA Zhi-yun,PAN Shan-shan,SHEN Yu-jun,HAO Zhe,GE Jun,WANG Kai.Application of Cardiac Molecular Marker NT-proBNP in the Assessment of Cardioprotective Effect of Exercise Preconditioning[J].China Sport Science,2011,31(12):55-61. |
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| Authors: | MA Zhi-yun PAN Shan-shan SHEN Yu-jun HAO Zhe GE Jun WANG Kai |
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| Institution: | MA Zhi-yun,PAN Shan-shan,SHEN Yu-jun,HAO Zhe,GE Jun,WANG Kai Shanghai University of Sport,Shanghai 200438,China. |
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| Abstract: | Objective: On the basis of the cardioprotective effect of exercise preconditioning(EP),this study was designed to focus cardiac molecular marker NT-proBNP to comprehensively assess the protective effect of EP on the heart undergoing the exhaustive exercise by means of the associated application of NT-proBNP,cTnI and HBFP staining.Methods:One hundred SD rats were randomly assigned to control group,EE group,EP group and EP+EE group.The rat model of EP was set up by means of the single bout of intermittent tre... |
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| Keywords: | NT-proBNP exercise preconditioning cardioprotective effect exhaustive exercise rat |
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