Identification of acetic-acid tolerance of Saccharomyces cerevisiae strains by microsatellite markers |
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| Authors: | XIAO Yin HU Yun ZHANG Liang XUE Wei and SHI Gui-yang |
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| Institution: | XIAO Yin;HU Yun;ZHANG Liang;XUE Wei;SHI Gui-yang;The Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University;National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University;School of Information Science and Technology, Nanjing Agricultural University; |
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| Abstract: | The aim of this paper is to use the microsatellites to evaluate acid-tolerance in Saccharomyces(S.) cerevisiae. Microsatellites have been widely used as the molecular marker to classify and identify S. cerevisiae strains, analyze genetic relationships among strains, and reveal genetic diversity of S. cerevisiae populations. In this paper, 25 key microsatellites of S. cerevisiae from 44 industrial yeast strains are investigated in the medium withconcentration gradients of acetic acid. Based on the analysis of correlations between the key microsatellite loci repeat numbers and acid-tolerance of the strains, the allele size of 4P14 a and 10P13 is positively related to acid-tolerance(p ? 0.05), the allele size of AT-X, 4P1 a and 10P8 are significantly negatively related to acid-tolerance(p ? 0.01). The above results provide informations on the molecular biodiversity of S. cerevisiae strains and can be a theoretical guidance for molecular marker assisted selection. |
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| Keywords: | microsatellite Saccharomyces cerevisiae acetic acid-tolerance |
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