| 纤维素分解菌的分离、筛选及其环境适应性初步研究 |
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| 引用本文： | 孙晓华,罗安程.纤维素分解菌的分离、筛选及其环境适应性初步研究[J].科技通报,2005,21(2):236-241. |
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| 作者姓名： | 孙晓华 罗安程 |
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| 作者单位： | 浙江大学,环境与资源学院,杭州,310029 |
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| 基金项目： | 嘉兴市-浙江大学合作项目
国家人事部留学回国人员择优项目
国家教委留学回国人员基金 |
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| 摘 要： | 以滤纸平板和羧甲基纤维素钠培养基为基础培养基,从采集的样品中筛选出具有分解纤维素能力的38株菌株。采用纤维素刚果红培养基进行粗选,得到10株透明圈较大的菌株。将这10株菌株进行液体发酵培养,测定其酶活力,得到4株分解纤维素能力较强的菌株。对这4株菌株进行碳源、温度、pH值的适应性研究。结果发现,真菌3和真菌6的适应性都比较好。
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| 关 键 词： | 纤维素分解菌 纤维素 分离筛选 |
| 文章编号： | 1001-7119(2005)02-0236-06 |
| 修稿时间： | 2003年12月19 |
Isolation and Screening of Cellulose-decomposing Microorganisms and Their Adaptability to Some Environmental Factors |
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| SUN xiao-hua,LUO An-cheng.Isolation and Screening of Cellulose-decomposing Microorganisms and Their Adaptability to Some Environmental Factors[J].Bulletin of Science and Technology,2005,21(2):236-241. |
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| Authors: | SUN xiao-hua LUO An-cheng |
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| Abstract: | Filter paper and carboxylic methyl fiber atrium were used as media to isolate and screen cellulose-decomposing microorganisms, and 38 strains were isolated from samples of soil and compost prepared from chicken manure, swine manure and cow manure. Of the isolated 38 strains, 10 strains showed a great capacity in cellulose decomposing, indicat ed by large and distinct red circles around the colonies in Congo red agar medium. Four strains of fungi were obtained with highest cellulase activity using liquid medium. Study on the adaptability to carbon sources, temperature and pH showed that two strains have a great potential to be used as composting inoculums. |
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| Keywords: | cellulose-decomposing microorganism cellulose isolation and screening |
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