| 大花蕙兰快速繁殖技术的研究 |
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| 引用本文： | 鞠守勇,高小蛾,陈其国,李莉,高广斌.大花蕙兰快速繁殖技术的研究[J].武汉职业技术学院学报,2008,7(5):59-61. |
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| 作者姓名： | 鞠守勇 高小蛾 陈其国 李莉 高广斌 |
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| 作者单位： | 武汉职业技术学院,生物工程系,湖北,武汉,430074 |
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| 基金项目： | 2008年度湖北省教育厅科研计划项目(B类项目)"大花惠兰工厂化生产工艺的研究" |
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| 摘 要： | 本文对大花蕙兰外植体消毒方式，原球茎增殖的培养基，大花蕙兰无根茵诱导生根培养基三个方面进行了系统的研究。结果表明：外植体在用A．1％Tween-60的1％HgCl2溶液消毒8分钟效果最明显，利于原球茎增殖的培养基为MS＋6-BA1．0mg／L＋NAA0．5mg／L，增殖系数为3．97，最佳的生根培养基为MS＋NAA0．2mg／1＋GA1．0mg／1＋香蕉泥150g／L，生根率最高可以达到100％。
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| 关 键 词： | 大花蕙兰 快速繁殖 组织培养 |
Study on Rapid Multiplication Techniques for Cymbidium Hybridism |
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| JU Shou-yong,GAO Xiao-e,CHEN Qi-guo,LI Li,GAO Guang-bin.Study on Rapid Multiplication Techniques for Cymbidium Hybridism[J].Journal of Wuhan Institute of Technology,2008,7(5):59-61. |
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| Authors: | JU Shou-yong GAO Xiao-e CHEN Qi-guo LI Li GAO Guang-bin |
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| Institution: | JU Shou-yong,GAO Xiao-e,CHEN Qi-guo,LI Li,GAO Guang-bin(Wuhan Institute of Technology,Wuhan430074,China) |
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| Abstract: | In this paper,several crucial technical questions on the multiplication of Cymbidium Hybridism have been studied,including the method of disinfection,the best culture medium for multiplication of protocorm like-body(PLB),and the medium of rooting.The results are as follows: 1%HgCl2 solution with added 1%Tween-60 which has been treated for 8 minutes is most suitable for explants disinfection;The best medium for the propagation of PLB is MS+6-BA 1.0mg/L+NAA 0.5 mg/L,the propagation coefficient is 3.97,MS+NAA0... |
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| Keywords: | Cymbidium hybridism rapid multiplication tissue culture |
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