| 小麦中尿囊素的HPLC分析 |
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| 引用本文： | 回瑞华,刁全平,侯冬岩,李铁纯,吕丽,隋潇.小麦中尿囊素的HPLC分析[J].鞍山师范学院学报,2012,14(4):38-41. |
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| 作者姓名： | 回瑞华 刁全平 侯冬岩 李铁纯 吕丽 隋潇 |
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| 作者单位： | 1. 鞍山师范学院化学与生命科学学院,辽宁鞍山,114007
2. 大连理工大学化学工程与工艺学院,辽宁大连,116085 |
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| 基金项目： | 辽宁省教育厅科学技术基金资助课题(2024201050) |
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| 摘 要： | 采用高效液相色谱法对小麦中尿囊素含量进行测定.色谱柱为Kromasil C18,流动相为V甲醇∶V水=5∶95,柱温30℃,流速0.5 mL/min,HPLC测定尿囊素含量变异系数小于1.1%,回收率为95.80%～105.4%,最低检出限0.5μg/mL.
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| 关 键 词： | 小麦 尿囊素 高效液相色谱法 |
Determination of the Allantion from Wheat by HPLC |
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| Institution: | HUI Rui-hua1,DIAO Quan-ping1,HOU Dong-yan1,LI Tie-chun1,LV Li1,SHUI Xiao2(1.School of Chemistry and Life Science,Anshan Normal University,Anshan Liaoning 114007,China; 2.Chemical engineering and Technology College of Dalian University of Technology,Dalian Liaoning 116085,China) |
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| Abstract: | The contents of the allantion in wheats are determinated by HPLC.The column is kromasil C18,mobile phase is methanol:water(5∶95,V∶V),the flow rate is 0.5 mL/min,the column temperature is 35 ℃.The coefficient of variation of allantoin content is determined less than 1.1% by HPLC,the recovery is 95.80%~105.4%,the minimum detection limit is 0.5 μg/mL. |
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| Keywords: | Wheat Allantion HPLC |
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