| Intestinal microflora in rats with ischemia／reperfusion liver injury |
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| 作者姓名： | Xing HC Li LJ Xu KJ Shen T Chen YB Sheng JF Yu YS Chen YG |
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| 作者单位： | Key Laboratory of Infectious Diseases,Ministry of Public Health of China,Institute of Infectious Diseases,Department of Infectious Diseases,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou310003,China,Key Laboratory of Infectious Diseases,Ministry of Public Health of China,Institute of Infectious Diseases,Department of Infectious Diseases,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou310003,China,Key Laboratory of Infectious Diseases,Ministry of Public Health of China,Institute of Infectious Diseases,Department of Infectious Diseases,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou310003,China,Key Laboratory of Infectious Diseases,Ministry of Public Health of China,Institute of Infectious Diseases,Department of Infectious Diseases,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou310003,China,Key Laboratory of Infectious Diseases,Ministry of Public Health of China,Institute of Infectious Diseases,Department of Infectious Diseases,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou310003,China |
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| 基金项目： | 国家重点基础研究发展计划(973计划),中国博士后科学基金,浙江大学校科研和教改项目 |
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| 摘 要： | Objectives:To investigate the intestinal microflora status related to ischemia/reperfusion(I/R)liver injury and explorethe possible mechanism.Methods:Specific pathogen free grade Sprague-Dawley rats were randomized into three groups:Controlgroup(n=8),sham group(n=6)and I/R group(n=10).Rats in the control group did not receive any treatment,rats in the I/R groupwere subjected to 20 min of liver ischemia,and rats in the sham group were only subjected to sham operation.Twenty-two hourslater,the rats were sacrificed and liver enzymes and malondialdehyde(MDA),superoxide dismutase(SOD),serum endotoxin,intestinal bacterial counts,intestinal mucosal histology,bacterial translocation to mesenteric lymph nodes,liver,spleen,andkidney were studied.Results:Ischemia/reperfusion increased liver enzymes,MDA,decreased SOD,and was associated withplasma endotoxin elevation in the I/R group campared to those in the sham group.Intestinal Bifidobacteria and Lactobacillidecreased and intestinal Enterobacterium and Enterococcus
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| 关 键 词： | 肠道微生物群 局部缺血／多次灌注液 肝损伤 内毒素 细菌迁移 |
Intestinal microflora in rats with ischemia/reperfusion liver injury |
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| Xing HC,Li LJ,Xu KJ,Shen T,Chen YB,Sheng JF,Yu YS,Chen YG.Intestinal microflora in rats with ischemia/reperfusion liver injury[J].Journal of Zhejiang University Science,2005,6(1):14-21. |
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| Authors: | Xing Hui-chun Li Lan-juan Xu Kai-jin Shen Tian Chen Yun-bo Sheng Ji-fang Yu Yun-song Chen Ya-gang |
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| Institution: | Key Laboratory of Infectious Disease, Ministry of Public Health of China, Institute of Infectious Diseases, Department of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China. |
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| Abstract: | Objectives: To investigate the intestinal microflora status related to ischemia/reperfusion (I/R) liver injury and explore the possible mechanism. Methods: Specific pathogen free grade Sprague-Dawley rats were randomized into three groups: Control group (n=8), sham group (n=6) and I/R group (n=10). Rats in the control group did not receive any treatment, rats in the I/R group were subjected to 20 min of liver ischemia, and rats in the sham group were only subjected to sham operation. Twenty-two hours later, the rats were sacrificed and liver enzymes and malondialdehyde (MDA), superoxide dismutase (SOD), serum endotoxin,intestinal bacterial counts, intestinal mucosal histology, bacterial translocation to mesenteric lymph nodes, liver, spleen, and kidney were studied. Results: Ischemia/reperfusion increased liver enzymes, MDA, decreased SOD, and was associated with plasma endotoxin elevation in the I/R group campared to those in the sham group. Intestinal Bifidobacteria and Lactobacilli decreased and intestinal Enterobacterium and Enterococcus, bacterial translocation to kidney increased in the I/R group compared to the sham group. Intestinal microvilli were lost, disrupted and the interspace between cells became wider in the I/R group.Conclusion: I/R liver injury may lead to disturbance of intestinal microflora and impairment of intestinal mucosal barrier function,which contributes to endotoxemia and bacterial translocation to kidney. |
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| Keywords: | Ischemia/reperfusion (I/R) Liver injury Microflora Endotoxin Bacterial translocation |
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