Ripple structure-generated hybrid electrokinetics for on-chip mixing and separating of functionalized beads |
| |
| Authors: | I-Fang Cheng Sheng-Chuan Chiang Cheng-Che Chung Trai-Ming Yeh Hsien-Chang Chang |
| |
| Institution: | 1.National Nano Device Laboratories, National Applied Research Laboratories, Tainan, Taiwan;2.Department of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan;3.Institute of Nanotechnology and Microsystems Engineering, National Cheng Kung University, Tainan, Taiwan;4.Medical Laboratory Science and Biotechnology, National Cheng Kung University, Tainan, Taiwan;5.Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan, Taiwan |
| |
| Abstract: | We present an electrokinetics-based microfluidic platform that is capable of on-chip manipulating, mixing, and separating microparticles through adjusting the interrelated magnitudes of dielectrophoresis and AC electroosmosis. Hybrid electrokinetic phenomenon is generated from an electric field-induced micro-ripple structure made of ultraviolet-curable glue. Size-dependent particle separation and selective removal over the ripple structure is demonstrated successfully. Varying the waveform from sine-wave to square-wave allows generating a fluid convection at specific positions to mix the antibody-functionalized beads and antigen. Potential application in the bead-based immunoassay was also demonstrated for immuno-reaction and subsequently separating the bead-bead aggregate and non-binding beads on-chip. |
| |
| Keywords: | |
|
|
