| 蒺藜苜蓿液泡膜H^＋-ATPase A亚基cDNA的电子克隆与序列分析 |
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| 作者姓名： | 孙艳香 李平 |
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| 作者单位： | 廊坊师范学院,河北,廊坊,065000 |
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| 基金项目： | 廊坊师范学院博士专项基金 |
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| 摘 要： | 根据物种间同源基因相对保守的特点，利用生物信息学的方法，以大豆液泡膜H^＋-ATPaseA亚基基因cDNA序列为信息探针，通过电子克隆得到了蒺藜苜蓿液泡膜H^＋-ATPaseA亚基cDNA，命名为MtVHA-A。该cDNA长2750bp，其中含5’非翻译区220bp，开放阅读框1875bp，3’非翻译区655bp，其推断的编码产物由624个氨基酸组成，理论分子量为69kDa，与蜜柑、番茄、拟南芥、棉花等植物中液泡膜H^＋-ATPaseA亚基氨基酸序列同源性高达90％以上，且有很高的功能区段保守性。
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| 关 键 词： | 蒺藜苜蓿 液泡膜H^＋-ATPase A亚基 MtVHA-A |
In Silico Cloning and Analysis of Tonoplast H+-ATPase A subunit cDNA in Medicago truncatula |
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| Authors: | SUN Yan-xiang LI Ping |
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| Institution: | SUN Yan-xiang LI Ping |
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| Abstract: | In the present paper,a tonoplast H^＋ - ATPase A subunit cDNA,named MtVHA-A was cloned in Medicago truncatula by EST in silico cloning. The cDNA contained 2750 bp with 5＇ - UTR of 220 bp, ORF region of 1875 bp, 3＇ - UTR of 655 bp, and encoded a 69 kDa protein with 624 amino acid. The deduced amino acid sequence showed more than 90% similarity to homologues from other plants such as Citrus unshiu, Lycopersicon esculentum, Arabidopsis thaliana and Gossypium hirsutum, and highly conserved functional domains of the gene class. |
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| Keywords: | MtVHA-A |
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