| 不同蛇含委陵菜居群遗传多样性的ISSR分析 |
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| 引用本文： | 牛友芽,欧资东,赵溶娟,吴弼,喻娟,佘朝文.不同蛇含委陵菜居群遗传多样性的ISSR分析[J].怀化学院学报,2012(11):41-44. |
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| 作者姓名： | 牛友芽 欧资东 赵溶娟 吴弼 喻娟 佘朝文 |
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| 作者单位： | [1]怀化学院生命科学系／民族药用植物资源研究与利用湖南省重点实验室,湘西药用植物与民族植物学湖南省高校重点实验室,湖南怀化418008；,浙江杭州310052 [2]怀化学院浙江中南建设集团有限公司,浙江杭州310052 |
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| 基金项目： | 湖南省教育厅一般项目（09C754）;湖南省教育厅科技创新平台项目（09K105）;湖南省科技计划重点项目（2009FJ2008;2012H4064）. |
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| 摘 要： | 以8个蛇含委陵菜居群为材料进行ISSR分析.从20条ISSR引物中筛选出4条稳定性强、条带清晰及多态性丰富的引物;筛选出的ISSR引物共扩增出41条带,其中39条多态性带,多态性比率为95.21%.用Ntsys2.10e软件进行UPGMA聚类分析.结果表明:蛇含委陵菜种质资源具有较高的遗传多样性,且其遗传多样性差异与生态分布和地理来源密切相关.
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| 关 键 词： | 蛇含委陵菜 ISSR 遗传多样性 |
Genetic Diversity of Potentilla kleiniana from Different Populations Based on ISSR Analysis |
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| Institution: | NIU You - ya, OU Zi - dong, ZHAO Rong - juan, WU Bi, YU Juan, SHE Chao - wen( 1. Department of Life Science, Laboratory of Hunan Provincefor Study and Utilization of Ethnic Medicinal Plant Resources, Laboralory o/ Hunan Higher Education for Hunan - western Medicinal Plant and Ethnobotany, Huaihua University, Huaihua, Hunan 418008; 2. Zhongnan Construction Group Limited Company in Zhejiang, Hangzhou, Zhejiang 310052 ) |
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| Abstract: | 8 samples of Potentilla kleiniana from different areas were analyzed by ISSR markers. The confirmed 4 primers were screened out from 20 test primers, with highly reproducible, clear and polymorphic bands. The results showed that 39 （95.21%） polymorphic bands were obtained in the total of 41 bands. UPGMA dendrogram analyzed by NTSYS demonstrated that the diversity levels of the different PotentiUa kleiniana resources were relatively high, suggested the relationship of Potentilla kleiniana from different places were related with the geographical location and ecological distribution. |
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| Keywords: | Potentilla kleiniana ISSR analysis genetic diversity |
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