| 温度、pH及碳氮源对产色素青霉菌株（Penicilliumsp．）生长的影响 |
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| 引用本文： | 黄思梅,张镜.温度、pH及碳氮源对产色素青霉菌株（Penicilliumsp．）生长的影响[J].韩山师范学院学报,2010,31(6):57-63. |
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| 作者姓名： | 黄思梅 张镜 |
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| 作者单位： | 嘉应学院生命科学学院,广东梅州514015 |
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| 摘 要： | PSO312是从土壤分离的1个青霉菌株，生长中能产生不同酸碱性条件下显黄色与紫红色等天然色素物质．通过菌株固体平板上恒温培养，168h测定菌落直径的方法研究了温度、pH及碳、氮源对菌株（Penicilliumsp．）生长的影响．研究表明31℃为菌株生长的最适温度．31℃培养以pH2—3的最快，pH8—9生长次之，24℃培养以pH8—9生长最快，pH3—4其次．菌株以蛋白胨4g／L、牛肉膏4g／L、酵母膏2g／L及尿素2g／L为氮源培养的菌落直径分别为55．75mm、51．5mm、55．5mm及25．3mm．在（NH4）2SO4 2g／L、NH4NO3 3g／L、NaNO3 4g／L、KN034g／L用量之上培养生长最快，菌落直径分别为35mm、34mm、35mm、及32mm．葡萄糖、蔗糖用量30g／L、果糖20g／L及乳糖25g／L的生长速率最快，菌落直径分别为45mm、38mm、38mm及39mm．丙三醇与淀粉25g／L培养的菌落直径均为39mm．
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| 关 键 词： | 青霉属 天然色素 氮源 碳源 |
Effects of pH,Carbon Sources and Nitrogen Sources of Mediums and Incubation Temperature on the Growth of a Penicillium Strain |
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| HUANG Si-mei,ZHANG Jing.Effects of pH,Carbon Sources and Nitrogen Sources of Mediums and Incubation Temperature on the Growth of a Penicillium Strain[J].Journal of Hanshan Teachers College,2010,31(6):57-63. |
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| Authors: | HUANG Si-mei ZHANG Jing |
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| Institution: | (College of Life Science, Jiaying University, Meizhou 514015, China) |
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| Abstract: | PSO3 12 （Penicillium sp.） strain isolated from a soil, can produce yellow and purple natural pigments cultured with different pH media. The effects of temperature, pH value, Carbon sources and nitrogen sources on the strain growth was investigated according to the colony diameter after which was cultured on the agar plate media for 168h. The results showed that the strain growth optimal temperature was 31 ℃. The strain cultured at 31 ℃ grew the fastest on pH 2-3 media, the second on pH 8~9 media. And then the strain cultured at 24 ℃ grew the fastest on pH 8~9 media, the second on pH 3~4 media. The colony diameters cultured on the media contained peptone 4 g/L, beef extract 4 g/L, yeast extract 2 g/L and urea, 2 g/L as organic nitrogen sources were 55.75 mm, 51.5 mm, 55.5 mm and 25.3 mm respectively. The colony diameters cultured on the media contained （NH4）2SO4 2 g/L, NH4NO3 3 g/L, NaNO3 4 g/L and KNO3 4 g/L as inorganic nitrogen sources were 35 ram, 34 mm, 35 mm and 32 mm respectively. The colony diameters cultured on the media contained glucose and sucrose 30g/L, fructose 20 g/L and lactose 25 g/L of the fastest growth rate, colony diameters of 45 ram, 38 mm, 38 mm and 39 mm. The strain on the dosages of glycerol and starch 25 g/L grew the fastest respectively. |
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| Keywords: | Penicillium sp natural pigment nitrogen sources carbon sources |
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