| 滇大蓟中黄酮的提取及其对羟基自由基清除作用研究 |
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| 引用本文： | 管春平,何艳娇,徐成东.滇大蓟中黄酮的提取及其对羟基自由基清除作用研究[J].楚雄师专学报,2013(6):50-55. |
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| 作者姓名： | 管春平 何艳娇 徐成东 |
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| 作者单位： | 楚雄师范学院化学与生命科学系,云南楚雄675000 |
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| 摘 要： | 以乙醇为提取剂,用浸提法提取滇大蓟中的黄酮,用Fenton法初步探究提取液清除羟基自由基的能力,并与BHT（二丁基羟基甲苯）溶液和芦丁溶液进行比较。结果表明,乙醇浸提法提取滇大蓟中黄酮的最佳条件为：60%的乙醇溶液,料液比为1∶30（g/mL）,提取温度为80℃,提取时间为4h,在此条件下,黄酮提取得率为0.163%;同浓度同体积的滇大蓟提取液、芦丁溶液和BHT溶液对羟基自由基（.OH）的清除效果为：滇大蓟提取液〉芦丁溶液〉BHT溶液。
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| 关 键 词： | 滇大蓟 黄酮 提取 羟基自由基 清除 |
Extraction of flavonoids from Cirsium chlorolepsis and its role of hydroxyl radical scavenging |
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| Institution: | GUAN Chun - ping; HE Yah - jiao; XU Cheng - dong (Department of chemistry and life science, Chuxiong Normal University, Chuxiong 675000, China) |
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| Abstract: | Flavonoids are extracted with ethanol from Cirsium chlorolepsis and its abilities of scaven- ging hydroxyl radical （ OH ） are detected by Fenton method as compared with BHT （butylated hydroxytoluene） solution and rutin solution. The results show that the optimum extraction conditions are： taking 60% ethanol as extracting agent, ratio of material to liquid is 1：30 （g/mL）, extraction tem- perature is 80℃, extraction time for 4h. Under that condition, the yield rate of flavonoids is 0. 163%. The extent of scavenging hydroxyl radical for the three solutions in the same concentration and volume are ： Cirsium chlo is extractant 〉 rutin solution 〉 BHT solution. |
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| Keywords: | Cirsium chlo /s flavonoids extraction hydroxyl radical scavenging effect |
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