| 实时BIA技术对蛋白A与免疫球蛋白G相互作用的研究 |
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| 作者姓名： | 裴仁军 杨秀荣 汪尔康 |
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| 作者单位： | 中国科学院长春应用化学研究所电分析化学开放研究实验室, 国家电化学和光谱研究中心 长春 130022 |
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| 基金项目： | 国家自然科学基金资助项目!(200075027) |
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| 摘 要： | 应用实时BIA技术,探讨了蛋白A与小鼠免疫球蛋白G (MIgG)之间的相互作用,求出相互作用的动力学速率常数ka=5.07×104 (mol/L)-1s-1,kd=9.65×10-5(s-1),结合常数KA=5.25×108(mol/L)-1.同时使用蛋白A固定的传感片用于MIgG浓度的检测,在 0.64～ 12mg/L浓度区间内,MIgG的响应值与其浓度有非常好的线性关系.
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| 关 键 词： | 实时BIA技术 蛋白A 免疫球蛋白G 动力学常数 |
| 收稿时间： | 2000-11-15 |
Studies on Interaction between Protein A and Immunoglobul in G Using Real-Time BIA Technique |
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| Authors: | Pei Renjun Yang Xiurong Wang Erkang |
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| Institution: | Laboratory of Electroanalytical Chemistry and National Analytical Research Center of Electrochemistry and Spectroscopy, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 |
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| Abstract: | The interaction betw een protein A and MIgG has been investigated.Some interesting kinetic parameters have been obtained.The kinetic constants between protein A and MIgG that we have obtained f rom the BIA senso rg ram is as follow ing :associate rate constant :ka=5.07×104 (mol/L)-1s-1, dissociate rate constant :kd=9.65×10-5(s-1), af finity constant :KA=5.25×108(mol/L)-1.The concentrat ion detection of MIgG, using protein A immobilized sensor chip, has been also studied.A good linear relationship betw een SPR response unit s and MIgG concent ration has been found in the range of 0.64 ～ 12mg/ L. |
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| Keywords: | Real-time BIA technique protein A immunoglobulin G kinetic constants |
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