| 三叶半夏组培苗的病毒检测 |
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| 引用本文： | 郭巧萍,唐香山,陈集双.三叶半夏组培苗的病毒检测[J].科技通报,2010,26(1):104-108. |
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| 作者姓名： | 郭巧萍 唐香山 陈集双 |
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| 作者单位： | 1. 南京工业大学,生物与制药工程学院,南京,211810;浙江理工大学,生物工程研究所,杭州,310018
2. 浙江理工大学,生物工程研究所,杭州,310018 |
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| 基金项目： | 国家“863”项目(2002AA24112A) |
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| 摘 要： | 组织培养阶段植物细胞中的病毒含量较低，难以用常规方法进行可靠检测。本研究采用DAS-ELISA、RT-PCR和核酸杂交三种方法对不同来源的12个三叶半夏（Pinellia ternata （Thunb．）Breit．）组培苗样品中的黄瓜花叶病毒（Cucumber mosaic virus，CMV）和大豆花叶病毒（Soybean mosaic virus，SMV）进行比较检测。结果显示：经DAS-ELISA检测，4个样品携带CMV，4个样品携带SMV，1个样品同时携带2种病毒；经RT-PCR检测。5个样品携带CMV，6个样品携带SMV，1个样品同时携带2种病毒；经核酸杂交检测验证，其结果与RT-PCR的检测结果一致。可见，对于三叶半夏组培苗，DAS-ELISA检测灵敏度不够高，需用灵敏度更高和特异性更强的核酸杂交方法来对其进行病毒检测，确保检测结果准确可靠。
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| 关 键 词： | 三叶半夏 组培苗 黄瓜花叶病毒 大豆花叶病毒 病毒检测 |
Detection of Viruses from Tissue-culturing Plantlets of Pinellia ternata (Thunb.) Breit. |
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| GUO Qiaoping,TANG Xiangshan,CHEN Jishuang.Detection of Viruses from Tissue-culturing Plantlets of Pinellia ternata (Thunb.) Breit.[J].Bulletin of Science and Technology,2010,26(1):104-108. |
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| Authors: | GUO Qiaoping TANG Xiangshan CHEN Jishuang |
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| Institution: | 1.College of Biotechnology and Pharmaceutical Engineering/a>;Nanjing University of Technology/a>;Nanjing 211810/a>;China/a>;2.Institute of Bioengineering/a>;Zhejiang Sci-Tech University/a>;Hangzhou 310018/a>;China |
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| Abstract: | The concentration of viruses in plant cells are induced during tissue culturing periods,and it is difficult to use conventional methods for reliable detection.We used DAS-ELISA,RT-PCR and nucleic acid hybridization after PCR amplification to test Cucumber mosaic virus(CMV) and Soybean mosaic virus(SMV) from tissue-culturing plantlets of P.ternata.Among twelve seedling lines,CMV was detected from four seedling lines,SMV was detected from four seedling lines,while we found one seedling line was complexly infe... |
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