Preparation of protein samples for gel electrophoresis by sequential extraction |
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| Authors: | Zhong Bo-xiong Weng Hong-biao Fang Wei-huan |
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| Institution: | (1) College of Animal Sciences, Zhejiang University, 310029 Hangzhou, China |
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| Abstract: | Since preparation and solubilization of protein samples are crucial factors in proteome research, the authors established
a sequential extraction technique to prepare protein samples from the body wall of the 5th instar larvae of silkworm,Bombyx mori. Two kinds of protein samples were obtained from the body wall using the method. Between the two types of samples only about
15% proteins were identical; the majority were different, indicating that more species of proteins could be obtained with
the sequential extraction method; which will be useful for preparation of protein samples for proteome study. |
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| Keywords: | Proteome Sequential extraction 2D-electrophoresis Protein spot match Amino acid sequence |
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