| HBV核酸光敏生物素探针的制备 |
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| 引用本文： | 李弘华.HBV核酸光敏生物素探针的制备[J].孝感职业技术学院学报,2003,6(3):71-73. |
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| 作者姓名： | 李弘华 |
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| 作者单位： | 湖北职业技术学院护理系 湖北孝感432000 |
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| 摘 要： | 利用设计合成的特异性引物，通过PCR扩增获得乙肝病毒的S区621个bpDNA片段．该扩增产物经EcoRI与BamHI双酶切后直接克隆进入PSK载体进行表达，再通过扩增重组菌株，以获得大量的S区DNA片段，对它们进行光敏生物素标记，制成探针，对乙肝病毒进行检测灵敏度高，特异性强，其灵敏度可达到4pg水平。
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| 关 键 词： | HBV 核酸光敏生物素探针 特异性引物 PCR 乙肝 病毒 灵敏度 |
| 文章编号： | 1671-8178(2003)03-0071-03 |
| 修稿时间： | 2003年6月5日 |
The Preparation of HBV Nucleic Acid Photoactive Biotin Probe |
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| LI Hong-hua.The Preparation of HBV Nucleic Acid Photoactive Biotin Probe[J].Journal of Xiaogan Vocational-Technical College,2003,6(3):71-73. |
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| Authors: | LI Hong-hua |
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| Abstract: | To utilize the synthesized idiosyncratic primer, the 621 bpDNA fragments in S-district of HBV can be obtained by the amplification of PCR. After the product is cut by enzyme of EcoRI and BamHI, it is directly cloned and expressed in the PSK vehicle, then is amplified into the recombined strain, to obtain many fragments of DNA in S-district of HBV. If they are marked with photoactive biotin and become probe, it will be high sensitive and strongly idiosyncratic to detect the HBV. The sensitivity can reach 4pg level. |
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| Keywords: | PCR amplification HBV DNA clone marked with Photoactive Biotin nucleic acid probe |
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