| Improvement of the efficacy of influenza vaccination （H5N1） in chicken by using extract of Cochinchina momordica seed （ECMS） |
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| 作者姓名： | Rajput ZI Xiao CW Hu SH Arijo AG Soomro NM |
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| 作者单位： | [1]Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China [2]Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tando Jam 70060, Pakistan |
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| 基金项目： | Projeci （No. 2004C32047） supported by the Department of Science and Technology of Zhejiang Province, China ACKN0WLEDGEMENT Higher Education Commission of Pakistan is appreciated for financial support of a Ph.D. scholarship to Rajput Zahid Iqbal （first author）. |
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| 摘 要： | Seeds of a Chinese traditional medicine plant, Cochinchina momordica were used in the present study for the improvement of influenza vaccine （HSN 1） in chicken. Crude extraction from Cochinchina momordica seed （ECMS） was obtained by ethanol extraction method. In experiment No. 1, two weeks old chickens were immunized with influenza vaccine （HSN1） alone or combined with ECMS （5, 10, 20, 40 and 80 μg/dose）. Serum IgG antibody levels （by ELISA） as well as effects on dally weight gain were measured on 0, 7, 14 and 28th day after immunization. Results revealed that all ECMS groups numerically increased the antibody levels while 10 and 20 μg/dose groups significantly （P〈0.05） enhanced total IgG antibody on day 28, when compared with control. Average daily weight gain was also significantly higher in 20 μg/dose ECMS group. Adjuvant effect was also confirmed in experiment No. 2 when chickens were immunized with 20 μg/dose ECMS and antibody titer was measured through hemagglutination inhibition （HI）. It is concluded that ECMS has potential to improve the immune responses and deserve further study as an adjuvant.
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| 关 键 词： | 鸡 H5N1禽流感 疫苗接种效果 木鳖子种子提取物 佐剂 |
| 收稿时间： | 2006-09-19 |
| 修稿时间： | 2007-01-25 |
Improvement of the efficacy of influenza vaccination (H5N1) in chicken by using extract of <Emphasis Type="Italic">Cochinchina momordica</Emphasis> seed (ECMS) |
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| Rajput ZI,Xiao CW,Hu SH,Arijo AG,Soomro NM.Improvement of the efficacy of influenza vaccination (H5N1) in chicken by using extract of Cochinchina momordica seed (ECMS)[J].Journal of Zhejiang University Science,2007,8(5):331-337. |
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| Authors: | Rajput Zahid Iqbal Xiao Chen-wen Hu Song-hua Arijo Abdullah G Soomro Noor Mohammad |
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| Institution: | (1) Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, 310029, China;(2) Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tando Jam, 70060, Pakistan |
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| Abstract: | Seeds of a Chinese traditional medicine plant, Cochinchina momordica were used in the present study for the improvement of influenza vaccine (H5N1) in chicken. Crude extraction from Cochinchina momordica seed (ECMS) was obtained by ethanol extraction method. In experiment No. 1, two weeks old chickens were immunized with influenza
vaccine (H5N1) alone or combined with ECMS (5, 10, 20, 40 and 80 μg/dose). Serum IgG antibody levels (by ELISA) as well as
effects on daily weight gain were measured on 0, 7, 14 and 28th day after immunization. Results revealed that all ECMS groups
numerically increased the antibody levels while 10 and 20 μg/dose groups significantly (P<0.05) enhanced total IgG antibody on day 28, when compared with control. Average daily weight gain was also significantly
higher in 20 μg/dose ECMS group. Adjuvant effect was also confirmed in experiment No. 2 when chickens were immunized with
20 μg/dose ECMS and antibody titer was measured through hemagglutination inhibition (HI). It is concluded that ECMS has potential
to improve the immune responses and deserve further study as an adjuvant.
Project (No. 2004C32047) supported by the Department of Science and Technology of Zhejiang Province, China |
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| Keywords: | Cochinchina momordica ECMS Adjuvant Chicken Avian influenza (H5N 1) Bird flue |
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