| 双吡啶分光光度法测定小麦植酸含量研究 |
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| 引用本文： | 时侠清,沙超,姚维东,王超.双吡啶分光光度法测定小麦植酸含量研究[J].安徽科技学院学报,2009,23(6):10-14. |
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| 作者姓名： | 时侠清 沙超 姚维东 王超 |
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| 作者单位： | 安徽科技学院,植物科学学院,安徽,凤阳,233100;安徽科技学院,植物科学学院,安徽,凤阳,233100;安徽科技学院,植物科学学院,安徽,凤阳,233100;安徽科技学院,植物科学学院,安徽,凤阳,233100 |
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| 基金项目： | 安徽省教育厅自然基金项目 |
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| 摘 要： | 本文建立了用硫酸铁铵-双吡啶比色法定量测定小麦植酸含量的一种新方法.在所述试验条件下,样品重为25mg,加热时间为30min能较好的测定出籽粒中的植酸含量,5次重复测定3个样品的相对标准偏差在0.92%～2.68%之间.内标试验表明,植酸的平均回收率约为97.3%,测定结果与三氯化铁比色法进行相关分析达极显著水平.因此,该方法可用于小麦籽粒中植酸含量的快速测定.
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| 关 键 词： | 小麦 植酸 双吡啶比色法 含量 |
Study on Bipyridine Colorimetric Method for Determination of Wheat Phytic Acid Content |
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| SHI Xia-qing,SHA Chao,YAO Wei-dong,WANG Chao.Study on Bipyridine Colorimetric Method for Determination of Wheat Phytic Acid Content[J].Journal of Anhui Science and Technology University,2009,23(6):10-14. |
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| Authors: | SHI Xia-qing SHA Chao YAO Wei-dong WANG Chao |
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| Institution: | SHI Xia-qing,SHA Chao,YAO Wei-dong,WANG Chao(Department of Botany,Anhui Science , Technology University,Fengyang 233100,China) |
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| Abstract: | The paper established a new way that the content of wheat grain phytic acid was determined by anllnoniilln ferric sulfate-bipyridine colorimetric method.It would get a good result under the experimental conditions in which the sample weight is 25 mg and the heating time is 30 minutes.The coefficient variation was between 0.92%-2.68% on 5 trials in 3 samples.The internal standard experiment showed that the average return-ratio of phytic acid was 97.3% approximately.The determination result was significant di... |
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| Keywords: | Wheat Phytic acid Bipyridine colorimetric method Content |
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