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| 伴发ATTR型淀粉样沉积的骨关节炎患者的临床和生物学特征分析(英文)简 | |
| 作者姓名： | Ya-jun GU Peng GE Yun MU Jin-hai LU Fang ZHENG Xu-guo SUN |
| 摘 要： | Objective： Our aim was to investigate clinical and laboratory characteristics of osteoarthritic patients who had amyloid deposition in their knee joints. Methods： Synovial membranes were obtained from 36 patients with knee osteoarthritis （OA） who underwent joint replacement surgery. From this sample, the diagnosis of amyloid was deter- mined by Congo red staining, which demonstrated apple-green birefringence under a polarized microscope. All syn- ovial membranes were immunohistochemically characterized for the expressions of amyloid immunoglobulin light chain （AL-K and AL-,k）, serum amyloid-A （SAA）, amyloidogenic transthyretin （ATTR）, and amyloidogenic 152- microglobulin （A152M）. Matrix-assisted laser desorption-ionizaton/time of flight mass spectrometry （MALDI-TOF MS） was used to analyze transthyretin （TTR） isoforms in the serum of each patient. Results： Nine cases （25%） were found to be amyloid-positive. Immunohistochemicaliy, eight cases （88.9%） had ATTR deposition, and one sample （11.1%） was shown to be AL-K-positive. MALDI-TOF MS identified that the TTR in the serum of the patients was unmodified wild-type TTR, TTR-Cys-S-S-Cys, and TTR-Cys-S-S-CysGly. The age at surgery and the disease duration were sig- nificantly higher in the ATTR-positive group than in the ATTR-negative group. Knee score and function score were significantly lower in the ATTR-positive group than in the ATTR-negative group. Conclusions： Amyloid deposition in synovial membranes of OA patients was found to be ATTR and AL-K. TTR in the serum of the patients was unmodified wild-type TTR together with two isoforms. The high age at surgery, long disease duration, and a deteriorated knee function were associated with ATTR amyloid deposition in the osteoarthritic knee joints. |
| 关 键 词： | Amyloid Immunoglobulin light chain Matrix-assisted laser desorption-ionizaton/time of flight massspectrometry （MALDI-TOF MS） Osteoarthritis Transthyretin |
| 收稿时间： | 2013 Feb 9 |
Clinical and laboratory characteristics of patients having amyloidogenic transthyretin deposition in osteoarthritic knee joints |
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| Ya-jun GU,Peng GE,Yun MU,Jin-hai LU,Fang ZHENG,Xu-guo SUN.Clinical and laboratory characteristics of patients having amyloidogenic transthyretin deposition in osteoarthritic knee joints[J].Journal of Zhejiang University Science,2014,15(1):92-99. | |
| Authors: | Ya-jun Gu Peng Ge Yun Mu Jin-hai Lu Fang Zheng Xu-guo Sun |
| Institution: | 1. School of Medical Laboratory, Tianjin Medical University, Tianjin, 300203, China 2. Department of Clinical Laboratory, Tianjin Medical University Cancer Institute and Hospital, Tianjin, 300060, China 3. Department of Clinical Laboratory, Tianjin Children’s Hospital, Tianjin, 300074, China |
| Abstract: | ObjectiveOur aim was to investigate clinical and laboratory characteristics of osteoarthritic patients who had amyloid deposition in their knee joints.MethodsSynovial membranes were obtained from 36 patients with knee osteoarthritis (OA) who underwent joint replacement surgery. From this sample, the diagnosis of amyloid was determined by Congo red staining, which demonstrated apple-green birefringence under a polarized microscope. All synovial membranes were immunohistochemically characterized for the expressions of amyloid immunoglobulin light chain (AL-κ and AL-λ), serum amyloid-A (SAA), amyloidogenic transthyretin (ATTR), and amyloidogenic β2-microglobulin (Aβ2M). Matrix-assisted laser desorption-ionizaton/time of flight mass spectrometry (MALDI-TOF MS) was used to analyze transthyretin (TTR) isoforms in the serum of each patient.ResultsNine cases (25%) were found to be amyloid-positive. Immunohistochemically, eight cases (88.9%) had ATTR deposition, and one sample (11.1%) was shown to be AL-κ-positive. MALDI-TOF MS identified that the TTR in the serum of the patients was unmodified wild-type TTR, TTR-Cys-S-S-Cys, and TTR-Cys-S-S-CysGly. The age at surgery and the disease duration were significantly higher in the ATTR-positive group than in the ATTR-negative group. Knee score and function score were significantly lower in the ATTR-positive group than in the ATTR-negative group.ConclusionsAmyloid deposition in synovial membranes of OA patients was found to be ATTR and AL-κ. TTR in the serum of the patients was unmodified wild-type TTR together with two isoforms. The high age at surgery, long disease duration, and a deteriorated knee function were associated with ATTR amyloid deposition in the osteoarthritic knee joints. |
| Keywords: | Amyloid Immunoglobulin light chain Matrix-assisted laser desorption-ionizaton/time of flight mass spectrometry (MALDI-TOF MS) Osteoarthritis Transthyretin |
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