A facile approach to construct hybrid multi-shell calcium phosphate gene particles |
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| Authors: | Zhi-xue Xu Ran Zhang You-xiang Wang and Qiao-ling Hu |
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| Institution: | (1) Division of Engineering, Brown University, 182 Hope Street, Providence, RI 02912 |
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| Abstract: | The calcium phosphate (CAP) particles have attracted much attention in gene therapy.How to construct stable gene particles was the determining factor.In this study,hybrid multi-shell CaP gene particles were successfully constructed.First,CaP nanoparticles served as a core and were coated with DNA for colloidal stabilization.The ζ-potential of DNA-coated CaP nanoparticles was-15 mV.Then polyethylenimine (PEI) was added and adsorbed outside of the DNA layer due to the electrostatic attraction.The ζ-potential of hybrid multi-shell CaP particles was slightly positive.With addition of PEI,the hybrid multi-shell particles could condense DNA effectively,which was de-termined by ethidium bromide (EtBr) exclusion assay.The hybrid particles were spherical and uniform with diameters of about 150 nm at proper conditions.By simple modification of PEI,the hybrid multi-shell CaP gene particles were successfully constructed.They may have great potential in gene therapy. |
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| Keywords: | Calcium phosphate Polyethylenimine (PEI) Hybrid Gene |
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