Electrotaxis of lung cancer cells in ordered three-dimensional scaffolds |
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| Authors: | Sun Yung-Shin Peng Shih-Wei Lin Keng-Hui Cheng Ji-Yen |
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| Institution: | 1Research Center for Applied Sciences, Academia Sinica, Taipei City 11529, Taiwan;2Institute of Biophotonics, National Yang-Ming University, Taipei City 11221, Taiwan;3Institute of Physics, Academia Sinica, Taipei City 11529, Taiwan;4Department of Mechanical and Mechantronic Engineering, National Taiwan Ocean University, Keelung 20224, Taiwan |
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| Abstract: | In this paper, we report a new method to incorporate 3D scaffold with electrotaxis measurement in the microfluidic device. The electrotactic response of lung cancer cells in the 3D foam scaffolds which resemble the in vivo pulmonary alveoli may give more insight on cellular behaviors in vivo. The 3D scaffold consists of ordered arrays of uniform spherical pores in gelatin. We found that cell morphology in the 3D scaffold was different from that in 2D substrate. Next, we applied a direct current electric field (EF) of 338?mV/mm through the scaffold for the study of cells’ migration within. We measured the migration directedness and speed of different lung cancer cell lines, CL1-0, CL1-5, and A549, and compared with those examined in 2D gelatin-coated and bare substrates. The migration direction is the same for all conditions but there are clear differences in cell morphology, directedness, and migration speed under EF. Our results demonstrate cell migration under EF is different in 2D and 3D environments and possibly due to different cell morphology and/or substrate stiffness. |
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