人CD34+干/祖细胞异常表达基因hCLP46功能的初步探讨 |
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作者姓名: | 张恬 常乃柏 王友信 刘辉 杜杰 宋曼殳 卢小梅 王嵬 刘利新 |
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作者单位: | 1.中国科学院研究生院生物系 北京100049 ;2.卫生部北京医院血液科 北京100730 ;3.新疆医科大学第一附属医院 新疆乌鲁木齐 830054 ; |
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摘 要: | hCLP46是从MDS-AML的CD34+细胞cDNA文库中筛选出的一个新的基因,为了进一步对该基因进行功能、性质研究,本实验利用RT-PCR技术对该基因进行了组织特异性表达检测,并将该基因构建到真核表达载体pcDNA3.1(+)中,转染U937细胞,发现过量表达该基因的U937细胞能够抑制TGF-61538;对p15基因的上调。此外,利用生物信息学手段对该基因进行序列分析,得到其启动子区存在一个典型的“CpG island”。提示该基因有可能参与甲基化调节途径。通过本文的研究,为进一步研究该基因的作用机制打下基础。
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关 键 词: | hCLP46 骨髓增生异常综合征(MDS) CpG island p15 |
Function study of hCLP46 from human CD34+ stem/progenitor cells |
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Authors: | ZHANG Tian CHANG Nai-Bai WANG You-Xin LIU Hui DU Jie SONG Man-Shu LU Xiao-Mei WANG Wei LIU Li-Xin |
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Institution: | 1.Department of Biology, Graduate University of Chinese Academy of Science. ;BeiJing 100049 ;2.Department of Hematology, BeiJing Hospital, Ministry of Health. BeiJing 100730 ;3.First hospital affiliated to XinJiang Medical University. Urumchi 830054 ; |
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Abstract: | hCLP46, a novel gene, has been screened out from the cDNA library of MDS-AML patient’s CD34+ stem cell. In order to further study the biological characters of the gene, RT-PCR was used to test the tissue specificity of hCLP46 gene expression. The results revealed hCLP46 was expressed in adult brain, not in fetal liver. After being sequenced, hCLP46 gene was cloned into XbaI~BamHI sites of pcDNA3.1. pcDNA3.1-hCLP46-myc was further transient transfected into U937 cells to detect the mechanisms of cell proliferation. It was shown that the expression of p15 inducted by TGF-βwas down regulated .By reference to gene sequence, the bio-informatics approach was used to elucidate the biology function of hCLP46, the analysis showed that a classical “CpG island” was found in the promoter of the gene which implied that the gene might be participated in methylation pathway. |
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Keywords: | hCLP46 Myelodysplastic syndrome (MDS) CpG island P15 |
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