| RP-HPLC法同时测定金匮肾气片中黄芩苷和丹皮酚的含量 |
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| 引用本文： | 付恩桃,刘修树,李会婷,范高福,胥振国,方丽波.RP-HPLC法同时测定金匮肾气片中黄芩苷和丹皮酚的含量[J].宜春学院学报,2020(3):24-26. |
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| 作者姓名： | 付恩桃 刘修树 李会婷 范高福 胥振国 方丽波 |
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| 作者单位： | 合肥职业技术学院生物应用技术系;中国科学技术大学附属第一医院 |
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| 基金项目： | 安徽省高校自然科学基金项目(KJ2019A1117,KJ2019A1112,KJ2016A614,KJ2016A614);合肥职业技术学院校级自然科学重点项目(201714KJA005);合肥职业技术学院校级质量工程项目“名师工作坊”(2018MSGZF002);安徽省高校省级质量工程项目(2017jyxm0672)。 |
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| 摘 要： | 目的:建立同时测定金匮肾气片中黄芩苷和丹皮酚含量的高效液相色谱法。方法:采用C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-0.1%磷酸水溶液(52:48),检测波长278 nm,柱温30℃,流速1.0 mL/min。结果:黄芩苷在0.485~19.406μg/mL,丹皮酚在1.079~43.157μg/mL线性关系良好。平均回收率(n=9)分别为99.6%,100.5%,含量测定结果表明,不同批次金匮肾气片之间黄芩苷和丹皮酚含量水平差异不大。结论:经方法学验证,该法可用于金匮肾气片中黄芩苷和丹皮酚含量的快速测定。
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| 关 键 词： | 金匮肾气片 黄芩苷 丹皮酚 高效液相色谱法 |
Simultaneous Determination of Baicalin and Paeonol in Jingui Shenqi Tablets by RP-HPLC |
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| FU En-tao,LIU Xiu-shu,LI Hui-ting,FAN Gao-fu,XU Zhen-guo,FANG Li-bo.Simultaneous Determination of Baicalin and Paeonol in Jingui Shenqi Tablets by RP-HPLC[J].Journal of Yichun University,2020(3):24-26. |
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| Authors: | FU En-tao LIU Xiu-shu LI Hui-ting FAN Gao-fu XU Zhen-guo FANG Li-bo |
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| Institution: | (Department of Biotechnology Applications,Hefei Vocational and Technology College,Hefei 238000,China;The First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230036,China) |
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| Abstract: | Objective:To establish a high performance liquid chromatography method for simultaneous determination of baicalin and paeonol in Jingui Shenqi tablets.Method:C18 column(4.6 mm×250 mm,5μm)was used with methanol-0.1%phosphoric acid aqueous solution(52:48).The detection wavelength is 278 nm,the column temperature is 30℃,and the flow rate is 1.0 mL/min.Results:Baicalin ranges from 0.485 to 19.406μg/mL,and paeonol is linear in the range of 1.079 to 43.157μg/mL.The average recovery rate(n=9)is 99.6%and 100.5%,respectively.The results shows that there was no significant difference in the levels of baicalin and paeonol between different batches.Conclusion:The method can be used to confirm the rapid determination of baicalin and paeonol in Jingui Shenqi tablets. |
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| Keywords: | Jingui Shenqi tablets baicalin paeonol high performance liquid chromatography |
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