| 利用高效液相色谱法测定茶叶中的儿茶素 |
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| 引用本文： | 郭佳莉,李国清,辛梅华,罗京,王育龄.利用高效液相色谱法测定茶叶中的儿茶素[J].泉州师范学院学报,2010,28(6):55-57,62. |
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| 作者姓名： | 郭佳莉 李国清 辛梅华 罗京 王育龄 |
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| 作者单位： | [1]泉州师范学院化学与生命科学学院,福建泉州362000 [2]华侨大学材料科学与工程学院,福建厦门361011 |
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| 基金项目： | 福建省自然科学基金资助项目，福建省科技计划项目，福建省高校服务海西重点建设项目，福建省泉州市科技计划项目 |
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| 摘 要： | 采用超声波提取茶叶中的茶多酚,并应用高效液相色谱法对不同品种的茶叶及同一产地不同档次的茶叶中的茶多酚成份进行分析测定.结果表明,采用Hyper-sil ODS柱,超纯水、甲醇和0.36%的乙酸为流动相,梯度洗脱,流速为0.8 mL.min-1,柱温40℃,在280 nm波长处可对儿茶素进行检测,其线性范围为0.6~3.0 g.L-1,R为0.999 6~0.999 9.
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| 关 键 词： | 高效液相色谱 茶叶 茶多酚 儿茶素 |
Determine Content of Catechins in Different Tea by HPLC Method |
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| GUO Jia-li,LI Guo-qing,Xin Mei-hua,LUO Jing,WANG Yu-ling.Determine Content of Catechins in Different Tea by HPLC Method[J].Journal of Quanzhou Normal College,2010,28(6):55-57,62. |
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| Authors: | GUO Jia-li LI Guo-qing Xin Mei-hua LUO Jing WANG Yu-ling |
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| Institution: | 1.School of Chemistry and Life Science,Quanzhou Normal University,Fujian 362000,China;2.College of Material Science and Engineering,Huaqiao University,Fujian 361011,China) |
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| Abstract: | The tea polyphenols of different type and grade teas were distilled by ultrasonic method.And high performance liquid chromatography（HPLC） was applied to rapidly determination of the standard catechins.The results show that the optimum chromatographic conditions are as follow：Hyper-sil ODS column,superpure water,methanol and 0.36% acetic acid as mobile phase,grads flow wash rate 0.8 mL·min-1,the column temperature 40 ℃,at 280 nm band could detect the catechins.Its linearity is kept in the range from 0.6—3.0 g·L-1（R=0.999 6—0.999 9）.The stability of this method is well and the recycle rate is high. |
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| Keywords: | HPLC tea tea polyphenols catechins |
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