| 量子点与胶体金之间的荧光共振能量转移用于免疫球蛋白测定的研究 |
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| 引用本文： | 李杰,徐诺,蔡齐勇,乔准,张琳.量子点与胶体金之间的荧光共振能量转移用于免疫球蛋白测定的研究[J].安阳师范学院学报,2013(5):6-11. |
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| 作者姓名： | 李杰 徐诺 蔡齐勇 乔准 张琳 |
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| 作者单位： | 郑州大学化学与分子工程学院,河南郑州450001 |
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| 基金项目： | 国家自然科学基金项目（No.J1210060）;河南省教育厅科学技术研究重点项目（13A150455）. |
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| 摘 要： | 结合量子点与胶体金颗粒优良的光学性能,利用抗体识别技术,基于人IgG修饰的量子点与羊抗人IgG修饰的胶体金之间的荧光共振能量转移效应,建立了一种新型免疫分析方法用于人IgG的检测.这种方法操作简便,实验条件温和,特异性好,安全可靠.
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| 关 键 词： | 免疫分析 人IgG 量子点 胶体金 荧光共振能量转移 |
Study of Human IgG Detection by Using Fluorescence Resonance Energy Transfer Between Quantum Dots and Gold Nanoparticles |
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| LI Jie,XU Nuo,CAI Qi- yong,QIAO Zhun,ZHANG Lin.Study of Human IgG Detection by Using Fluorescence Resonance Energy Transfer Between Quantum Dots and Gold Nanoparticles[J].Journal of Aayang Teachers College,2013(5):6-11. |
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| Authors: | LI Jie XU Nuo CAI Qi- yong QIAO Zhun ZHANG Lin |
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| Institution: | (College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China) |
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| Abstract: | Taking advantages of quantum dots and gold nanoparficles, utilizing the technology of antibody - an-tigen recognition, a new immune analysis method build up for the analysis of human IgC based on fluorescence resonance energy transfer between quantum dots and gold nanoparticles. This method could be carried out eas-ily and specifically under safe conditions. |
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| Keywords: | Immunoassay Human IgG Quantum dots Colloidal gold Fluorescence resonance energy trans-fer |
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