| 保藏温度、时间及代次对蛹虫草菌种质量的影响 |
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| 引用本文： | 方华舟,董海波,肖习明,左雪枝.保藏温度、时间及代次对蛹虫草菌种质量的影响[J].荆门职业技术学院学报,2011,26(2):5-10. |
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| 作者姓名： | 方华舟 董海波 肖习明 左雪枝 |
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| 作者单位： | 方华舟,左雪枝(荆楚理工学院生物工程学院,湖北,荆门,448000);董海波(北京中农绿亨种子科技有限公司,北京,100094);肖习明(荆门市农业局,湖北,荆门,448000) |
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| 基金项目： | 湖北省荆门市科技局研究与推广项目"蛹虫草(北冬虫夏草)引进与推广" |
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| 摘 要： | 目的：探索不同保藏温度、时间及菌种代次对蛹虫草菌种质量的影响。方法：以4℃、10℃、20℃等不同保藏温度,以30 d、60 d、90 d、180 d及360 d等不同保藏时间,以F1、F2、F3、F4等不同代次母种,以F0为对照组,对比菌种质量情况。结果：菌种质量由高到低依次为：10℃保藏1个月、4℃保藏1个月、20℃保藏1个月、4℃保藏2个月、4℃保藏3个月、10℃保藏2个月,其他基本无菌种价值;F1〉F2〉F3,F4基本无菌种价值。结论：4℃保藏1～2个月为佳,10℃保藏1个月为佳,菌种传代以3代以内为佳。
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| 关 键 词： | 蛹虫草菌种 保藏条件 角变率 菌丝状况 产量 |
Impact of Preservation Temperature,Time and Passages on the Quality of Cordyceps Species |
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| FANG Hua-zhou,DONG Hai-bo,XIAO Xi-ming,et al..Impact of Preservation Temperature,Time and Passages on the Quality of Cordyceps Species[J].Journal of Jingmen Vocational Technical College,2011,26(2):5-10. |
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| Authors: | FANG Hua-zhou DONG Hai-bo XIAO Xi-ming |
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| Institution: | FANG Hua-zhou1,DONG Hai-bo2,XIAO Xi-ming3,et al.(1 School of Bioengineering,Jingchu University of Technology,Jingmen,Hubei,448000,China,2 Beijing Zhongnong Lvhengseeds Sci-Technology Limited Company,Beijing,100094,3 Jingmen Municipal Bureau of Agriculture,China) |
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| Abstract: | Objective：To explore the impact of different preservation temperature,time and passages on the quality of Cordyceps species.Methods：To compare the quality of the Cordyceps species with different storage temperatures of 4 ℃,10 ℃,20 ℃,different storage time of 30 d,60 d,90 d,180 d and 360 d,different passages mother of F1,F2,F3,F4,control group of F0.Results：The quality of species indicate as following：10 ℃ 1 month,4 ℃ 1 month,20 ℃ 1 month,4 ℃ 2 months,4 ℃ 3 months,10 ℃ 2 months.Other species are non-value.F1 is the best,F2 is better than F3,F4 has almost no strain value.Conclusion：Preserving Cordyceps species for 1 to 2 months at 4 ℃ or 1 month at 10 ℃ is best,the strains of Cordyceps pass on from generation to generation better within 3 generations. |
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| Keywords: | Cordyceps species preservation conditions angle variability mycelium status yield |
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