一株产环糊精葡萄糖基转移酶的枯草芽孢杆菌的选育及产酶条件的研究 |
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引用本文: | 朱德艳.一株产环糊精葡萄糖基转移酶的枯草芽孢杆菌的选育及产酶条件的研究[J].荆门职业技术学院学报,2009,24(5):29-32. |
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作者姓名: | 朱德艳 |
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作者单位: | 荆楚理工学院,生物工程学院,湖北,荆门,448000 |
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基金项目: | 荆楚理工学院科学研究项目 |
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摘 要: | 介绍了一种从土壤中选育一株产环糊精葡萄糖基转移酶(CGTase)的芽孢杆菌的方法。经筛选,最终找到一株高产CGTase的菌株SC3—2,鉴定该菌株为嗜碱性枯草芽孢杆菌。通过对其产酶条件的探索,结果表明:以玉米淀粉为碳源,以黄豆粉为氮源,在pH值为6.5,30℃的条件下培养36h,菌株所产CG-Tase的酶活力可迭5046U/mL。
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关 键 词: | 嗜碱性枯草芽孢杆菌 菌种选育 环糊精葡萄糖基转移酶 产酶条件 |
Breeding and Enzymatic Properties of a Bacillus Subtilis Producing Cyclomaltodextrin Glucanotransferase |
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Institution: | ZHU De -yan ( Jingchu University of Technology, Jingrnen, Hubei ,448000, China) |
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Abstract: | An Alkaliphilic Bacillus Subtilis SC3 -2 with high Cycloinaltodextrin Glucanotransferase (CGTase)activity was screened and identified on the basis of physical characterization. The optimized conditions of CGTase production were obtained: maize starch,Soy flottr,30 ℃ ,pH 6.5,36 h,5 046 U/mL CGTase activity was achieved using submerged fermentation. |
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Keywords: | Alkaliphilic Bacillus Subtilis breeding Cyclomahodextrin Ghicanotransferase enzymatic properties |
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