| 经多聚赖氨酸修饰后的云母表面形貌及细胞DNA的AFM观察 |
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| 引用本文： | 宋发奎,张坤,齐浩.经多聚赖氨酸修饰后的云母表面形貌及细胞DNA的AFM观察[J].西安文理学院学报,2011(2):14-18. |
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| 作者姓名： | 宋发奎 张坤 齐浩 |
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| 作者单位： | 陕西师范大学生命科学学院,陕西西安710062 |
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| 基金项目： | 中央高校基本科研业务费专项资金资助项目（GK200902033） |
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| 摘 要： | 通过原子力显微镜(AFM)观察并分析多聚赖氨酸在不同浓度和不同修饰方式下云母表面的形貌,选择适于观察细胞DNA的最佳修饰条件.使用不同浓度的多聚赖氨酸对云母表面进行直接滴加和镀膜两种方式进行处理后,AFM观察其对云母表面形貌的影响以及DNA分子在修饰后的云母表面沉积情况.结果显示:多聚赖氨酸直接滴加至云母上时,其表面会...
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| 关 键 词： | 原子力显微镜 多聚赖氨酸 镀膜 DNA |
The AFM Observation of the Morphology of Mica Surface and Cellular DNA Modified by Poly-lysine |
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| SONG Fa-kui,ZHANG Kun,QI Hao.The AFM Observation of the Morphology of Mica Surface and Cellular DNA Modified by Poly-lysine[J].Journal of Xi‘an University of Arts & Science:Natural Science Edition,2011(2):14-18. |
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| Authors: | SONG Fa-kui ZHANG Kun QI Hao |
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| Institution: | (College of Life Science,Shaanxi Normal University,Xi＇an 710062,China) |
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| Abstract: | This study aims to investigate the optimal observation condition by atomic force microscope（AFM） for cellular DNA through the AFM observation and analysis of the morphology of mica surface under the condition of different concentrations of poly-lysine modification.Our method is to make an AFM observation of the morphology of mica surface and the deposition of cellular DNA on mica when poly-lysine of different concentrations is directly added to or coated on freshly cleaved mica.The result shows that particles of various size and aggregation were visible when poly-lysine was directly added to the mice.However,the number and granularity of particles and the roughness（Ra） were significantly reduced after poly-lysine was coated on the mica.The surface of mica coated with poly-lysine was smoother.The roughness was decreased with the lower concentration of poly-lysine.The roughness did not show a significant change when the concentration of poly-lysine was not higher than 0.1 μg/mL.DNA molecular were better spread when the surface of mica was coated with 0.1 μg/mL poly-lysine.The study concludes that,compared with the method of direct adding,the coating of poly-lysine was more effective for reducing the roughness.When coated with 0.1 μg/mL poly-lysine,DNA molecular were better spread on the surface of mica. |
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| Keywords: | Atomic Force Microscopy poly-lysine coating DNA |
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