| 基于共价连接的辣根过氧化物酶的直接电化学与高稳定性传感器构建 |
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| 引用本文： | 刘传银,李成艳.基于共价连接的辣根过氧化物酶的直接电化学与高稳定性传感器构建[J].郧阳师范高等专科学校学报,2010,30(3):18-22. |
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| 作者姓名： | 刘传银 李成艳 |
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| 作者单位： | 1. 郧阳师范高等专科学校,化学系,湖北,丹江口,442700
2. 房县第二中学,湖北,房县,442100 |
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| 基金项目： | 湖北省教育厅优秀中青年项目，湖北省教育厅高等学校教学研究项目，郧阳师范高等专科学校科研项目 |
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| 摘 要： | 利用金电极上自组装的3-巯基丙酸自组装膜,通过DCC和NHS共价连接辣根过氧化物酶（HRP）,构建了一种稳定性好、寿命长的过氧化氢电化学生物传感器.HRP在该电极上实现了直接电子传递,其氧化还原峰电位差为250mV,式量电位为-231 mV.该传感器对过氧化氢表现出良好的电催化活性,还原峰电流与过氧化氢含量在1.5-1000μmol/L之间呈现良好的线性关系,灵敏度达到1185μA/mM,检出限为0.5μmol/L.该传感器具有良好的稳定性、重现性,已用于合成样品中过氧化氢含量的测定,准确度良好.
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| 关 键 词： | 3-巯基丙酸自组装膜 辣根过氧化物酶 直接电化学 过氧化氢生物传感器 |
Direct Electrochemistry Horseradish Peroxides and Highly Stable Hydrogen Peroxide Biosensors Based on Covalent Immobilization on 3-mercaptopropionic Acid Self-assembled Monolayer |
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| LIU Chuan-yin,LI Cheng-yan.Direct Electrochemistry Horseradish Peroxides and Highly Stable Hydrogen Peroxide Biosensors Based on Covalent Immobilization on 3-mercaptopropionic Acid Self-assembled Monolayer[J].Journal of Yunyang Teachers College,2010,30(3):18-22. |
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| Authors: | LIU Chuan-yin LI Cheng-yan |
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| Institution: | 1. Chemistry Department, Yunyang Teachers＇ College, Danjiangkou 442700, China; 2. No. Two Middle School, Fangxian County 442100, China) |
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| Abstract: | A highly stable hydrogen peroxide biosensor has been prepared based on horseradish peroxides （HRP） covalently immobilized on 3--propionic acid self--assembled monolayer by DCC and NHS. Direct electrochemistry of HRP has been achieved with redox peak separation of 250 mV and formal potential of --231 inV. The proposed biosensor exhibits better electrocatalysis towards hydrogen peroxide, the linear range is 1.5--1000 μmol/L with sensitivity of 1185 μA/mM and detection limit of 0.5 μmol/L. The proposed biosensor shows its high stability, reproducibility and anti--interference, and has been used to determine hydrogen peroxide in synthesized samples with satisfactory results. |
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| Keywords: | 3--Mercaptopropionic acid self--assembled monolayer Horseradish peroxides direct electrochemistry Hydrogen peroxide biosensor |
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