| 高效土著花生根瘤菌筛选的初步研究 |
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| 引用本文： | 刘世旺,;刘建英,;徐艳霞,;徐君驰.高效土著花生根瘤菌筛选的初步研究[J].黄冈师范学院学报,2014(3):19-23. |
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| 作者姓名： | 刘世旺 ;刘建英 ;徐艳霞 ;徐君驰 |
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| 作者单位： | [1]黄冈师范学院生命科学学院,湖北黄州438000; [2]黄冈市蔬菜办公室,湖北黄州438000 |
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| 基金项目： | 湖北省教育厅高校产学研合作资助项目（No.C2010061）资助. |
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| 摘 要： | 利用YMA培养基,从新鲜花生根瘤中分离得到黄冈土著花生根瘤菌6株,分别编号为HG-1、HG-2、HG-3、HG-4、HG-5、HG-6。选取黄冈地区广泛使用的3个花生品种(红花1号、红花2号、中花1号)对6个菌株进行盆栽回接实验,结果显示:接种根瘤菌的花生植株都有结瘤,所有菌株均为花生根瘤菌。通过比较根瘤数量和质量,初步筛选出与花生品种红花1号、红花2号、中花1号具有最佳共生固氮匹配关系的根瘤菌菌株分别是HG-5、HG-3、HG-2。
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| 关 键 词： | 根瘤菌 花生 共生固氮 筛选 |
Preliminary study on screening of efficient indigenous peanut rhizobia |
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| Institution: | LIU Shi-wang, LIU Jian-ying, XU Yan-xia, XU Jun-chi (1.College of Life Science, Huanggang Normal University, Huangzhou 438000, Hubei, China; 2.Huanggang City Vegetable Administration Office, Huangzhou 438000, Hubei, China) |
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| Abstract: | 6 strains of indigenous rhizobia in the area of Huanggang have been isolated from fresh peanut nodule extracts with YMA medium.They were separately named as HG-1,HG-2,HG-3,HG-4,HG-5 and HG-6.The isolated strains were inoculated back onto 3 varieties of peanut plants （Honghua-1,Honghua-2,Zhonghua-1）that are widely sowed in Huanggang.The results of the experiment in sand bottles show that all the peanut plants can nodulate after being inocula-ted with the strains,and all the isolated strains are peanut rhizobia.By comparing quantity and weight of nodule,it is known that the strain HG-5 symbiotic nitrogen-fixing effect matches best with the peanut plant Honghua-1.So does HG-3 with Honghua-2,and HG-2 with Zhonghua-1. |
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| Keywords: | rhizobium peanut symbiotic nitrogen-fixing screening |
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