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Application of Box-Behnken design in optimization of allelopathic effects of Potamogeton pectinatus against Microcystis aeruginosa |
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| Authors: | Zhang Nan Ji Min Pan Tao Sun Changhong |
| Institution: | 1. Beijing Municipal Research Institute of Environmental Protection, National Engineering Research Center of Urban Environmental Pollution Control, Beijing 100037, China 2. School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China 3. Beijing Municipal Research Institute of Environmental Protection, National Engineering Research Center of Urban Environmental Pollution Control, Beijing 100037, China;School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China |
| Abstract: | Allelopathic effects of submerged macrophytes against algae are affected by many environmental factors which can only be measured one by one by traditional methods. Box-Behnken design of response surface methodology was used to optimize three environmental factors (temperature, light intensity and total dissolved solids) of allelopathic effects of Potamogeton pectinatus against Microcystis aeruginosa at the same time. By solving the regression equation and analyzing the response surface contour plots, the optimal conditions of the relatively inhibitory rate of Microcystis aeruginosa were that the temperature was 23°C, the light intensity was 2,700,lx and the total dissolved solids were 4,415,mg/L. Under these conditions, the optimal value of relatively inhibitory rate of Microcystis aeruginosa was 81.9%. According to validation experiments, the results of analysis indicated that the experimental values fitted well with the predicted ones. |
| Keywords: | allelopathic effects Box-Behnken design temperature light intensity total dissolved solids |
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