| 两种分光光度法测定金钱草中多糖含量的研究 |
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| 引用本文： | 刘俊劭,林志銮.两种分光光度法测定金钱草中多糖含量的研究[J].洛阳师范学院学报,2011,30(2):48-50. |
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| 作者姓名： | 刘俊劭 林志銮 |
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| 作者单位： | 武夷学院高校绿色化工技术重点实验室,福建武夷山,354300 |
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| 基金项目： | 武夷学院校科研基金项目(XQL05022) |
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| 摘 要： | 采用3,5-二硝基水杨酸法和苯酚-硫酸法分别对溶剂法提取分离的金钱草叶的粗多糖进行分析,考察两种分析方法的可靠性和准确性.实验结果表明,多糖含量测定结果分别为11.70%和11.36%.平行测定6次,3,5-二硝基水杨酸法测定样品加标回收率在92.52%~104.49%之间,RSD%=3.98%,检出限为0.022 mg/mL;苯酚-硫酸法测定样品加标回收率在93.29%~114.82%之间,RSD%=3.36%,检出限为0.018 mg/mL.两种方法都比较灵敏、可靠.
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| 关 键 词： | 多糖 金钱草 3 5-二硝基水杨酸 苯酚-硫酸 |
The Study on Two kinds of the Spectrophotometric Determination of Polysaccharide of Lysimachia Christinae Hance |
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| LIU Jun-shao,LIN Zhi-luan.The Study on Two kinds of the Spectrophotometric Determination of Polysaccharide of Lysimachia Christinae Hance[J].Journal of Luoyang Teachers College,2011,30(2):48-50. |
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| Authors: | LIU Jun-shao LIN Zhi-luan |
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| Institution: | LIU Jun-shao,LIN Zhi-luan (The Key Laboratory for Green Chemical Engineering and Technology,Wuyi University,Wuyishan 354300,China) |
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| Abstract: | Solvent extraction of Lysimachia christinae Hance's polysaccharide,is analyzed respectively with 3,5-dinitrosalicylic acid(DNS) and Phenol-Sulfuric Acid Colorimeter.The reliability and accuracy are of these two analysis methods are inspected.The results show that polysaccharide content was 11.70%.and11.36%.Parallel measurement is done 6 times;Rate of recovery with DNS method is between 92.52%~104.49%,RSD%=3.98%.The minimum detection limit is 0.022mg/mL.Rate of recovery of Phenol-Sulfuric Acid methods'is bet... |
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| Keywords: | polysaccharide Lysimachia christinae Hance 3 5-dinitrosalicylic acid phenol-sulfuric acid |
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