| 苦参不同器官中的生物碱含量分析 |
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| 引用本文： | 任欣,于昉,闫建义,赵东利.苦参不同器官中的生物碱含量分析[J].大连大学学报,2006,27(4):79-81. |
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| 作者姓名： | 任欣 于昉 闫建义 赵东利 |
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| 作者单位： | 1. 大连大学,医学院,辽宁,大连,116622
2. 大连大学,动物中心,辽宁,大连116622 |
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| 摘 要： | 目的]探讨苦参不同器官中的生物碱含量,为该资源植物的合理应用提供依据.方法]用薄层色谱法(TLC)分析测定了辽宁产苦参不同器官中苦参碱及氧化苦参碱含量.结果]各类器官,除叶片外,氧化苦参碱含量均显著超过苦参碱含量;苦参碱含量以种子中的为最高,占干重的0.065%,其次为茎(0.034%)、根(0.016%)、叶(0.013%);氧化苦参碱含量以根最高,占干重的3.847%,其次为种子(1.982%)、茎(0.140%),叶中未检测到;粗老根(直径大于1 cm)与细嫩根(直径在0.5~1.0 cm)中的两种生物碱含量差别不大.结论]达到一定生长年限后,生物碱积累不再有明显增加而趋于饱和;大部分器官中,氧化苦参碱含量高于苦参碱含量;种子及茎更适合作为苦参碱的提取器官.
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| 关 键 词： | 苦参 苦参碱 氧化苦参碱 薄层层析 含量分析 |
| 文章编号： | 1008-2395（2006）04-0079-03 |
| 收稿时间： | 2005-11-03 |
| 修稿时间： | 2005年11月3日 |
Analysis of alkaloid content in different organs in Sophora flavescens |
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| REN Xin,YU Fang,YAN Jian-yi,ZHAO Dong-li.Analysis of alkaloid content in different organs in Sophora flavescens[J].Journal of Dalian University,2006,27(4):79-81. |
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| Authors: | REN Xin YU Fang YAN Jian-yi ZHAO Dong-li |
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| Abstract: | Objective] In order to right use Sophora flavescens plant resource,contents of matrine and oxymatrine of various organs of Sophora flavescens from Liaoning province were tested in the present paper.Methods] TLC method.Results] Matrine of seeds is the highest in content among all the tested organs,being up to 0.065% of dry weight,then stems (0.034%),roots(0.016%) and leaves(0.013%);The content of oxymatrine in roots is the highest,up to 3.847% of dry weight,and then seeds(1.982%) and stems(0.140%),no oxymatrine was detected in leaves.The alkaloid content is not significantly different between old rough roots more than 1.0cm in diameter and young thin ones of 0.5~1.0cm in diameter.Conclusions] Contents of alkaloids in Sophora flavescens plants will keep stabilization in mature plants although the plants still grow;Oxymatrine is more than matrine in most organs of Sophora flavescens;Seeds and stems are appropriate organs for matrine extraction. |
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| Keywords: | Sophora flavescens matrine oxymatrine TLC content analysis |
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