| 琼脂糖凝胶中DNA回收技术的探讨 |
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| 引用本文： | 周君,陈正凯,徐剑,陆小平.琼脂糖凝胶中DNA回收技术的探讨[J].常熟理工学院学报,2007,21(8):42-47. |
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| 作者姓名： | 周君 陈正凯 徐剑 陆小平 |
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| 作者单位： | 苏州大学,城市科学学院,江苏苏州,215123;苏州大学,城市科学学院,江苏苏州,215123;苏州大学,城市科学学院,江苏苏州,215123;苏州大学,城市科学学院,江苏苏州,215123 |
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| 摘 要： | 从琼脂糖凝胶电泳分离的条带中回收DNA是常用的分子生物学技术。本试验通过比较常用的几种DNA片段的回收方法,发现用改进的离心法回收琼脂糖凝胶中的DNA片段效果较好,成本低,操作简单。并探讨了回收大片段DNA时,回收率较低的原因。
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| 关 键 词： | 琼脂糖凝胶 DNA 回收技术 离心法 |
| 文章编号： | 1008-2794（2007）08-0042-06 |
| 收稿时间： | 2007-05-11 |
A Discussion on the Technology of DNA Recovery from Agrose Gel |
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| ZHOU Jun,CHEN Zheng-kai,XU Jian,LU Xiao-ping.A Discussion on the Technology of DNA Recovery from Agrose Gel[J].Journal of Changshu Institute of Technology,2007,21(8):42-47. |
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| Authors: | ZHOU Jun CHEN Zheng-kai XU Jian LU Xiao-ping |
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| Institution: | School of Urbanology,Suzhou University,Suzhou 215123,China |
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| Abstract: | The technology of DNA recovery from agrose gel is in common use in molecular biology.By comparing the efficiencies of these methods,the improved method of frozen/spin and direct elution is more convenient and economical than the traditional methods.At the same time,we probe into the reasons for low rate of recovery. |
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| Keywords: | Agarose gel electrophoresis DNA recovery technology spin and direct elution |
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